ArticlePlant molecular biology1995
Ethylene-regulated expression of a carnation cysteine proteinase during flower petal senescence.
Article in Plant molecular biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 155 citations in OpenAlex.
- CRISPR/Cas9-Mediated Editing ofFrontiers in plant science · 2022Article
- Genome and transcriptome-based characterization of high energy carbon-ion beam irradiation induced delayed flower senescence mutant in Lotus japonicus.BMC plant biology · 2021Article
- Proteome and Ubiquitome Changes during Rose Petal Senescence.International journal of molecular sciences · 2019Article
- Identification of Wheat Inflorescence Development-Related Genes Using a Comparative Transcriptomics Approach.International journal of genomics · 2018Article
- Dynamic transcriptomic analysis of the early response of female flowers of Populus alba × P. glandulosa to pollination.Scientific reports · 2017Article
- An Ethylene-Protected Achilles' Heel of Etiolated Seedlings for Arthropod Deterrence.Frontiers in plant science · 2016Article
- Flower senescence: some molecular aspects.Planta · 2014Review
- Article
- The Antioxidants Changes in Ornamental Flowers during Development and Senescence.Antioxidants (Basel, Switzerland) · 2013Review
- Identification and expression analysis of ERF transcription factor genes in petunia during flower senescence and in response to hormone treatments.Journal of experimental botany · 2011Article
- Differential expression of genes identified by suppression subtractive hybridization in petals of opening carnation flowers.Journal of experimental botany · 2010Article
- Transcriptional activation of a 37 kDa ethylene responsive cysteine protease gene, RbCP1, is associated with protein degradation during petal abscission in rose.Journal of experimental botany · 2009Article
- Cloning and characterization of a novel cysteine protease gene (HbCP1) from Hevea brasiliensis.Journal of biosciences · 2008Article
- Integrated signaling in flower senescence: an overview.Plant signaling & behavior · 2007Article
- Gene expression in opening and senescing petals of morning glory (Ipomoea nil) flowers.Plant cell reports · 2007Article
- Article
- Induced expression of oryzain alpha gene encoding a cysteine proteinase under stress conditions.Journal of plant research · 2007Article
- Upregulation of a tonoplast-localized cytochrome P450 during petal senescence in Petunia inflata.BMC plant biology · 2006Article
- Expression of proteinase inhibitor II proteins during floral development in Solanum americanum.Planta · 2004Article
- Gene expression during anthesis and senescence in Iris flowers.Plant molecular biology · 2003Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The senescence of carnation (Dianthus caryophyllus L.) flower petals is regulated by the phytohormone ethylene and is associated with considerable catabolic activity including the loss of protein. In this paper we present the molecular cloning of a cysteine proteinase and show that its expression is regulated by ethylene and associated with petal senescence. A 1600 bp cDNA was amplified by polymerase chain reaction using a 5'-specific primer and 3'-nonspecific primer designed to amplify a 1-aminocyclopropane-1-carboxylate synthase cDNA from reverse-transcribed stylar RNA. The nucleotide sequence of the cloned product (pDCCP1) was found to share significant homology to several cysteine proteinases rather than ACC synthase. A single open reading frame of 428 amino acids was shown to share significant homology with other plant cysteine proteinases including greater than 70% identity with a cysteine proteinase from Arabidopsis thaliana. Amino acids in the active site of cysteine proteinases were conserved in the pDCCP1 peptide. RNA gel blot analysis revealed that the expression of pDCCP1 increased substantially with the onset of ethylene production and senescence of petals. Increased pDCCP1 expression was also associated with ethylene production in other senescing floral organs including ovaries and styles. The pDCCP1 transcript accumulated in petals treated with exogenous ethylene within 3 h and treatment of flowers with 2,5-norbornadiene, an inhibitor of ethylene action, prevented the increase in pDCCP1 expression in petals. The temporal and spatial patterns of pDCCP1 expression suggests a role for cysteine proteinase in the loss of protein during floral senescence.
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