ArticleNucleic acids research1995
Double bands in DNA gel electrophoresis caused by bis-intercalating dyes.
Article in Nucleic acids research, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed.
- Effect of Flow Rate and Ionic Strength on the Stabilities of YOYO-1 and YO-PRO-1 Intercalated in DNA Molecules.The journal of physical chemistry. B · 2023Article
- Characterizing Single-Molecule Conformational Changes Under Shear Flow with Fluorescence Microscopy.Journal of visualized experiments : JoVE · 2020Article
- Anticooperative Binding Governs the Mechanics of Ethidium-Complexed DNA.Biophysical journal · 2019Article
- Shining a Spotlight on DNA: Single-Molecule Methods to Visualise DNA.Molecules (Basel, Switzerland) · 2019Review
- Article
- Determining if DNA Stained with a Cyanine Dye Can Be Digested with Restriction Enzymes.Journal of visualized experiments : JoVE · 2018Article
- Photobleaching of YOYO-1 in super-resolution single DNA fluorescence imaging.Beilstein journal of nanotechnology · 2017Article
- Sizing femtogram amounts of dsDNA by single-molecule counting.Nucleic acids research · 2016Article
- The fluorescence properties and binding mechanism of SYTOX green, a bright, low photo-damage DNA intercalating agent.European biophysics journal : EBJ · 2015Article
- Interference of ATP with the fluorescent probes YOYO-1 andYOYO-3 modifies the mechanical properties of intercalator-stained DNA confined in nanochannels.Mikrochimica acta · 2015Article
- Mixed confinement regimes during equilibrium confinement spectroscopy of DNA.The Journal of chemical physics · 2014Article
- Heterogeneous staining: a tool for studies of how fluorescent dyes affect the physical properties of DNA.Nucleic acids research · 2013Article
- Compression of the DNA substrate by a viral packaging motor is supported by removal of intercalating dye during translocation.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Mechanical and structural properties of YOYO-1 complexed DNA.Nucleic acids research · 2010Article
- Fluorescent single-stranded DNA binding protein as a probe for sensitive, real-time assays of helicase activity.Biophysical journal · 2008Article
- DNA mapping using microfluidic stretching and single-molecule detection of fluorescent site-specific tags.Genome research · 2004Article
- Characterization of DNA/lipid complexes by fluorescence resonance energy transfer.Biophysical journal · 2003Article
- Real-time observation of a single DNA digestion by lambda exonuclease under a fluorescence microscope field.Nucleic acids research · 2001Article
- Single- and double-strand photocleavage of DNA by YO, YOYO and TOTO.Nucleic acids research · 1996Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Many bis-intercalating dyes used for fluorescence detection of DNA in electrophoresis have been reported to give band-splitting and band-broadening, which results in poor resolution and a decreased detection sensitivity. We have studied the dimeric dye YOYO-1, and to some extent also TOTO-1 and EthD-1, and found that in complex with DNA these dyes give rise to two components with different electrophoretic mobilities. Electrophoresis experiments and spectroscopic measurements on the two components show that they differ in that the DNA molecules have different amounts of dye bound. Our results exclude that the extra bands are caused by intermolecular cross-linking. Incubation of the samples for increasing times before electrophoresis makes the bands move closer and closer to each other as the dye molecules become more homogeneously distributed among the DNA molecules. Finally, the two bands merge into one at an intermediate position. This equilibration process is extremely slow at room temperature (days), and is therefore not a practical method to eliminate band-splitting in routine analysis. However, we find that if the temperature is raised to 50 degrees C, the dye-DNA complexes equilibrate completely in only 2 h.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.