Evidence map›Paper›PMID 7630716›Full record

ArticleNucleic acids research1995

Cloning and characterisation of a nuclear, site specific ssDNA binding protein.

M P Smidt, B Russchen, L Snippe, J Wijnholds, G Ab

Open access · greenAbstract read
In one paragraph

Article in Nucleic acids research, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
0.8field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed, 36 citations in OpenAlex.

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  10. Active transport of the survival motor neuron protein and the role of exon-7 in cytoplasmic localization.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2003
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

M P SmidtLaboratory of Biochemistry, University of Groningen, The Netherlands.
B Russchen
L Snippe
J Wijnholds
G Ab
University of Groningen · NL

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Estradiol inducible, liver-specific expression of the apoVLDL II gene is mediated through the estrogen receptor and a variety of other DNA-binding proteins. In the present study we report the cloning and characterisation of a single-strand DNA binding protein that interacts with the lower strand of a complex regulatory site, which includes the major estrogen responsive element and a site that resembles the rat albumin site D (apoVLDL II site D). Based on its binding specificity determined with electro-mobility shift assays, the protein is named single-strand D-box binding factor (ssDBF). Analysis of the deduced 302 amino acid sequence revealed that the protein belongs to the heteronuclear ribonucleoprotein A/B family (hnRNP A/B) and resembles other known eukaryotic single-strand DNA binding proteins. Transient transfection experiments in a chicken liver cell-line showed that the protein represses estrogen-induced transcription. A protein with similar binding characteristics is present in liver nuclear extract. The relevance of the occurrence of this protein to the expression of the apoVLDL II gene is discussed.

Indexed as

Cloning, MolecularAmino Acid SequenceAnimalsBase SequenceBinding SitesChickensDNA-Binding ProteinsEscherichia coliGene ExpressionGene Transfer TechniquesLipoproteins, VLDLLiverMaleMolecular Sequence DataRecombinant Fusion ProteinsRegulatory Sequences, Nucleic AcidDNA-Binding ProteinsLipoproteins, VLDLRecombinant Fusion ProteinsRibonucleoproteinsRNA, Messengersingle-strand D-box binding protein, Gallus gallus

Identifiers

PMID7630716
PMCPMC307042
OpenAlexW2127350329

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.