ArticleThe Journal of experimental medicine1995
Analysis of mutations in immunoglobulin heavy chain variable region genes of microdissected marginal zone (MGZ) B cells suggests that the MGZ of human spleen is a reservoir of memory B cells.
Article in The Journal of experimental medicine, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 97 papers.
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Who cites it
97 citing papers in PubMed, 289 citations in OpenAlex.
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- Clinical relevance of the compartments and lymphocyte subsets in the human spleen.Cell and tissue research · 2025Article
- Multi-dimensional analysis of B cells reveals the expansion of memory and regulatory B-cell clusters in humans living in rural tropical areas.Clinical and experimental immunology · 2025Article
- B Cell Differentiation and the Origin and Pathogenesis of Human B Cell Lymphomas.Methods in molecular biology (Clifton, N.J.) · 2025Review
- Unswitched memory B cell deficiency in children with sickle cell disease and response to pneumococcal polysaccharide vaccine.American journal of hematology · 2024Article
- CD27 on human memory B cells-more than just a surface marker.Clinical and experimental immunology · 2023Review
- B cells in human lymphoid structures.Clinical and experimental immunology · 2022Review
- CD45RB Glycosylation and Ig Isotype Define Maturation of Functionally Distinct B Cell Subsets in Human Peripheral Blood.Frontiers in immunology · 2022Article
- Bioinformatics and system biology approaches to identify the diseasome and comorbidities complexities of SARS-CoV-2 infection with the digestive tract disorders.Briefings in bioinformatics · 2021Article
- Systematic memory B cell archiving and random display shape the human splenic marginal zone throughout life.The Journal of experimental medicine · 2021Article
- CD38 Defines a Subset of B Cells in Rainbow Trout Kidney With High IgM Secreting Capacities.Frontiers in immunology · 2021Article
- Innate B Cells: the Archetype of Protective Immune Cells.Clinical reviews in allergy & immunology · 2020Review
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- Host and parasite responses in human diffuse cutaneous leishmaniasis caused by L. amazonensis.PLoS neglected tropical diseases · 2019Article
- The formation of mutated IgM memory B cells in rat splenic marginal zones is an antigen dependent process.PloS one · 2019Article
- Dissecting Integrin Expression and Function on Memory B Cells in Mice and Humans in Autoimmunity.Frontiers in immunology · 2019Article
- A tyrosine sulfation-dependent HLA-I modification identifies memory B cells and plasma cells.Science advances · 2018Article
37 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The splenic marginal zone (MGZ), which surrounds the mantle zone (MTZ) in human splenic white pulp, contains a phenotypically and morphologically distinct population of B cells. The origin of MGZ B cells is uncertain. Whereas some experiments in rodents have suggested that they are a distinct cell lineage responsible for the immune response to T-independent type 2 antigens, others have suggested that they are memory B cells derived from a germinal center (GC) response. The progeny of a GC reaction is expected to have rearranged immunoglobulin (Ig) genes that are mutated. The distribution of mutations would be expected to reflect the selection of Ig by its affinity for antigen. We have analyzed rearranged Ig heavy chain variable region (VH) 6 and VH 4.21 genes in MGZ and MTZ B cells microdissected from frozen sections of human spleen to determine whether these genes have the properties of an affinity-selected memory B cell population. MTZ B cells contained germline Ig VH genes, confirming previous reports and providing an internal control for mutational analysis. MGZ B cells contained Ig VH genes that were mutated, showing that these cells had been subjected to a mutational mechanism characteristically active in the GC. The rearranged VH 6 genes showed patterns of mutation indicative of an antigen selection process, whereas the distribution of mutations in VH 4.21 genes was not characteristic of gene selection by conventional T-dependent antigen. Our studies provide the first evidence that the human splenic MGZ is a reservoir of memory B cells.
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