ArticleThe EMBO journal1995
Protein-protein interaction via PAS domains: role of the PAS domain in positive and negative regulation of the bHLH/PAS dioxin receptor-Arnt transcription factor complex.
Article in The EMBO journal, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
62 citing papers in PubMed, 190 citations in OpenAlex.
- The aryl hydrocarbon receptor: structure, signaling, physiology and pathology.Signal transduction and targeted therapy · 2026Review
- Structural basis for the ligand-dependent activation of heterodimeric AHR-ARNT complex.Nature communications · 2025Article
- Effects of the carcinogen TCDD on δ-catenin stability and tumorigenic potential in prostate cancer cells.American journal of cancer research · 2025Article
- Nuclear localization of STING1 competes with canonical signaling to activate AHR for commensal and intestinal homeostasis.Immunity · 2023Article
- Tumor-Suppressive Functions of the Aryl Hydrocarbon Receptor (AhR) and AhR as a Therapeutic Target in Cancer.Biology · 2023Review
- PP2A phosphatase inhibition is anti-fibrotic through Ser77 phosphorylation-mediated ARNT/ARNT homodimer formation.Scientific reports · 2021Article
- Transitional States in Ligand-Dependent Transformation of the Aryl Hydrocarbon Receptor into Its DNA-Binding Form.International journal of molecular sciences · 2020Article
- Molecular analysis of NPAS3 functional domains and variants.BMC molecular biology · 2018Article
- Article
- Risk for animal and human health related to the presence of dioxins and dioxin-like PCBs in feed and food.EFSA journal. European Food Safety Authority · 2018Article
- Construction and Development of a Cardiac Tissue-Specific and Hypoxia-Inducible Expression Vector.Advanced pharmaceutical bulletin · 2018Article
- Structural hierarchy controlling dimerization and target DNA recognition in the AHR transcriptional complex.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Structural modeling of the AhR:ARNT complex in the bHLH-PASA-PASB region elucidates the key determinants of dimerization.Molecular bioSystems · 2017Article
- 2,3,7,8 Tetrachlorodibenzo-p-dioxin-induced RNA abundance changes identify Ackr3, Col18a1, Cyb5a and Glud1 as candidate mediators of toxicity.Archives of toxicology · 2017Article
- Bioanalytical and instrumental screening of the uptake of sediment-borne, dioxin-like compounds in roach (Rutilus rutilus).Environmental science and pollution research international · 2016Article
- Deciphering Dimerization Modes of PAS Domains: Computational and Experimental Analyses of the AhR:ARNT Complex Reveal New Insights Into the Mechanisms of AhR Transformation.PLoS computational biology · 2016Article
- Transcription factors in microalgae: genome-wide prediction and comparative analysis.BMC genomics · 2016Article
- De-repression of CSP-1 activates adaptive responses to antifungal azoles.Scientific reports · 2016Article
- Article
- Article
2 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Gene regulation by dioxins is mediated by the dioxin receptor-Arnt heterodimer, a ligand generated complex of two basic helix-loop-helix (bHLH)/Per-Arnt-Sim (PAS) transcription factors. By using dioxin receptor chimeras where the dimerization and DNA binding bHLH motif has been replaced by a heterologous DNA binding domain, we have detected an ability of Arnt to interact with the dioxin receptor via the PAS domain in a mammalian 'hybrid interaction' system. By coimmunoprecipitation assays, we have confirmed the ability of PAS domains of the dioxin receptor and Arnt to mediate independent heterodimerization in vitro. Selectivity for PAS dimerization was noted in our hybrid interaction system, as dioxin receptor or Arnt PAS-mediated homodimers were not detected. Surprisingly, however, the PAS domain of Per could dimerize with both the dioxin receptor and Arnt subunits in vitro, and disrupt the ability of these subunits to form a DNA binding heterodimer. Moreover, ectopic expression of Per blocked dioxin signalling in mammalian cells. The PAS domains of the dioxin receptor and Arnt are therefore novel dimerizing regions critical in formation of a functional dioxin receptor-Arnt complex, while the PerPAS domain is a potential negative regulator of bHLH/PAS factor function.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.