ArticleThe EMBO journal1995
Conservative sorting of F0-ATPase subunit 9: export from matrix requires delta pH across inner membrane and matrix ATP.
Article in The EMBO journal, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 78 citations in OpenAlex.
- A dynamic displacement mechanism drives protein import into mitochondria.bioRxiv : the preprint server for biology · 2026Article
- Protein insertion into the inner membrane of mitochondria: routes and mechanisms.FEBS open bio · 2024Review
- The ER membrane complex (EMC) can functionally replace the Oxa1 insertase in mitochondria.PLoS biology · 2022Article
- Interplay between Mitochondrial Protein Import and Respiratory Complexes Assembly in Neuronal Health and Degeneration.Life (Basel, Switzerland) · 2021Review
- Role of the Mitochondrial Protein Import Machinery and Protein Processing in Heart Disease.Frontiers in cardiovascular medicine · 2021Review
- Mitochondrial membrane potential.Analytical biochemistry · 2018Review
- Plant Mitochondrial Inner Membrane Protein Insertion.International journal of molecular sciences · 2018Review
- Granzyme B enters the mitochondria in a Sam50-, Tim22- and mtHsp70-dependent manner to induce apoptosis.Cell death and differentiation · 2017Article
- A panoramic overview of mitochondria and mitochondrial redox biology.Toxicological research · 2014Article
- Interactions between peptidyl tRNA hydrolase homologs and the ribosomal release factor Mrf1 in S. pombe mitochondria.Mitochondrion · 2013Article
- Delivery of drugs and macromolecules to mitochondria.Advanced drug delivery reviews · 2007Review
- Mitochondrial protein import and human health and disease.Biochimica et biophysica acta · 2007Review
- The Oxa2 protein of Neurospora crassa plays a critical role in the biogenesis of cytochrome oxidase and defines a ubiquitous subbranch of the Oxa1/YidC/Alb3 protein family.Molecular biology of the cell · 2004Article
- Ribosome binding to the Oxa1 complex facilitates co-translational protein insertion in mitochondria.The EMBO journal · 2003Article
- Yeast Oxa1 interacts with mitochondrial ribosomes: the importance of the C-terminal region of Oxa1.The EMBO journal · 2003Article
- Localisation of the human hSuv3p helicase in the mitochondrial matrix and its preferential unwinding of dsDNA.Nucleic acids research · 2002Article
- Mba1, a novel component of the mitochondrial protein export machinery of the yeast Saccharomyces cerevisiae.The Journal of cell biology · 2001Article
- Oxa1p acts as a general membrane insertion machinery for proteins encoded by mitochondrial DNA.The EMBO journal · 2001Article
- Oxa1p, an essential component of the N-tail protein export machinery in mitochondria.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Analysis of the sorting signals directing NADH-cytochrome b5 reductase to two locations within yeast mitochondria.Molecular and cellular biology · 1997Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In an attempt to understand the mechanisms of sorting of mitochondrial inner membrane proteins, we have analyzed the import of subunit 9 of the mitochondrial F1F0-ATPase (Su9) from Neurospora crassa, an integral inner membrane protein. A chimeric protein was used consisting of the presequence and the first transmembrane domain of Su9 fused to mouse dihydrofolate reductase (preSu9(1-112)-DHFR). This protein attains the correct topology across the inner membrane (Nout-Cin) following import. The transmembrane domain becomes first completely imported into the matrix, where after processing of the presequence, it mediates membrane insertion and export of the N-terminal tail. Import and export steps can be experimentally dissected into two distinct events. Translocation of the N-terminal hydrophilic tail out of the matrix was blocked when the presequence was not processed, indicating an important role of the sequences and charges flanking the hydrophobic domain. Furthermore, export was supported by a delta pH and required matrix ATP hydrolysis. Thus the hydrophobic transmembrane domain operates as a membrane insertion signal and not as a stop-transfer signal. Our findings suggest that several aspects of this sorting process have been conserved from their prokaryotic ancestors.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.