ArticleThe EMBO journal1995
Reconstitution of interactions between tyrosine kinases and the high affinity IgE receptor which are controlled by receptor clustering.
Article in The EMBO journal, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 161 citations in OpenAlex.
- Review
- Article
- Dexamethasone Induces FcγRIIb Expression in RBL-2H3 Cells.The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology · 2012Article
- Article
- Regulation and function of syk tyrosine kinase in mast cell signaling and beyond.Journal of signal transduction · 2011Article
- The SCHOOL of nature: I. Transmembrane signaling.Self/nonself · 2010Article
- Article
- Fer kinase is required for sustained p38 kinase activation and maximal chemotaxis of activated mast cells.Molecular and cellular biology · 2002Article
- Mast cells. Receptors, secretagogues, and signaling.Clinical reviews in allergy & immunology · 2002Review
- Adaptor FYB (Fyn-binding protein) regulates integrin-mediated adhesion and mediator release: differential involvement of the FYB SH3 domain.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- Aberrant B cell receptor signaling from B29 (Igbeta, CD79b) gene mutations of chronic lymphocytic leukemia B cells.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Sequential requirements of the N-terminal palmitoylation site and SH2 domain of Src family kinases in the initiation and progression of FcepsilonRI signaling.Molecular and cellular biology · 2000Article
- A balance between positive and negative signals in cytotoxic lymphocytes regulates the polarization of lipid rafts during the development of cell-mediated killing.The Journal of experimental medicine · 2000Article
- Review
- Altered expression of tyrosine kinases of the Src and Syk families in human T-cell leukemia virus type 1-infected T-cell lines.Journal of virology · 1999Article
- syk protein tyrosine kinase regulates Fc receptor gamma-chain-mediated transport to lysosomes.The EMBO journal · 1998Article
- Btk/Tec kinases regulate sustained increases in intracellular Ca2+ following B-cell receptor activation.The EMBO journal · 1998Article
- Phosphatidylinositol-3,4,5-trisphosphate (PtdIns-3,4,5-P3)/Tec kinase-dependent calcium signaling pathway: a target for SHIP-mediated inhibitory signals.The EMBO journal · 1998Article
- Functional role for Syk tyrosine kinase in natural killer cell-mediated natural cytotoxicity.The Journal of experimental medicine · 1997Article
- Compartmentalized IgE receptor-mediated signal transduction in living cells.The Journal of cell biology · 1997Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
High affinity IgE receptor (Fc epsilon RI) signaling after contact with antigen occurs in response to receptor clustering. This paper describes methodology, based on vaccinia virus driven protein expression, for probing signaling pathways and its application to Fc epsilon RI interactions with the lyn and syk tyrosine kinases. Reconstitution of the complete tetrameric Fc epsilon RI receptor, lyn and syk in a non-hematopoietic 'null' cell line is sufficient to reconstruct clustering-controlled receptor tyrosine phosphorylation and activation of syk, without apparent requirement for hematopoietic specific phosphatases. The src family kinase lyn phosphorylates Fc epsilon RI in response to receptor clustering, resulting in syk binding to the phosphorylated Fc epsilon RI. Lyn also participates in the tyrosine phosphorylation and activation of syk in a manner which is dependent on phosphorylated Fc epsilon RI. Using overexpression of active and dominant negative syk proteins in a mast cell line which naturally expresses Fc epsilon RI, we corroborate syk's role downstream of receptor phosphorylation, and demonstrate that syk SH2 domains protect receptor ITAMs from ongoing dephosphorylation. Based on these results, we propose that receptor clustering controls lyn-mediated Fc epsilon RI tyrosine phosphorylation by shifting a balance between phosphorylation and dephosphorylation towards accumulation of tyrosine phosphorylated Fc epsilon RI. Fc epsilon RI tyrosine phosphorylation functions to bring syk into a microenvironment where it becomes tyrosine phosphorylated and activated, thereby allowing clustering to indirectly control syk activity.
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