ArticleThe EMBO journal1995
A novel protein kinase gene ssp1+ is required for alteration of growth polarity and actin localization in fission yeast.
Article in The EMBO journal, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 60 citations in OpenAlex.
- Temperature-specific regulation of the NDR kinase Orb6 by the MAPK Sty1 to promote heat stress resilience.Journal of cell science · 2026Article
- The phosphatase inhibitor Sds23 promotes symmetric spindle positioning in fission yeast.Cytoskeleton (Hoboken, N.J.) · 2020Article
- Protein Phosphatases in G1 Regulation.International journal of molecular sciences · 2020Review
- Whole-Genome Sequencing of Suppressor DNA Mixtures Identifies Pathways That Compensate for Chromosome Segregation Defects inG3 (Bethesda, Md.) · 2018Article
- Express yourself: how PP2A-B55Current genetics · 2018Review
- Mechanisms Connecting the Conserved Protein Kinases Ssp1, Kin1, and Pom1 in Fission Yeast Cell Polarity and Division.Current biology : CB · 2018Article
- Phosphatases Generate Signal Specificity Downstream of Ssp1 Kinase in Fission Yeast.Molecular and cellular biology · 2017Article
- Article
- Regulatory roles of phosphorylation in model and pathogenic fungi.Medical mycology · 2016Review
- Nitrogen regulates AMPK to control TORC1 signaling.Current biology : CB · 2015Article
- Mechanisms of expression and translocation of major fission yeast glucose transporters regulated by CaMKK/phosphatases, nuclear shuttling, and TOR.Molecular biology of the cell · 2015Article
- Ssp1 CaMKK: A Sensor of Actin Polarization That Controls Mitotic Commitment through Srk1 in Schizosaccharomyces pombe.PloS one · 2015Article
- Genetic and physical interaction of Ssp1 CaMKK and Rad24 14-3-3 during low pH and osmotic stress in fission yeast.Open biology · 2014Article
- Snf1-like protein kinase Ssp2 regulates glucose derepression in Schizosaccharomyces pombe.Eukaryotic cell · 2012Article
- Linkers of cell polarity and cell cycle regulation in the fission yeast protein interaction network.PLoS computational biology · 2012Article
- Nutrient limitations alter cell division control and chromosome segregation through growth-related kinases and phosphatases.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2011Review
- Specific biomarkers for stochastic division patterns and starvation-induced quiescence under limited glucose levels in fission yeast.The FEBS journal · 2011Article
- Fission yeast germinal center (GC) kinase Ppk11 interacts with Pmo25 and plays an auxiliary role in concert with the morphogenesis Orb6 network (MOR) in cell morphogenesis.The Journal of biological chemistry · 2010Article
- The STE20/germinal center kinase POD6 interacts with the NDR kinase COT1 and is involved in polar tip extension in Neurospora crassa.Molecular biology of the cell · 2006Article
- Systematic deletion analysis of fission yeast protein kinases.Eukaryotic cell · 2005Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Temperature-sensitive suppressor mutants were isolated from two fission yeast mutants defective in cell shape control: ppe1, encoding a type 2A-like protein phosphatase, and sts5, one of 11 staurosporine-supersensitive mutants. Complementation tests showed that suppression was due to two chromosomal loci, ssp1 and ssp2. Cells of the ssp1 mutant grown at the restrictive temperature arrested uniformly with an elongated cell body and a 2C content of DNA. Interestingly, these mutant cells grew only in a monopolar manner. At a specific point in the G2 phase of the cell cycle, wild-type cells exhibit a drastic alteration in growth polarity, from mono- to bipolar. This change coincides with the distribution of cortical actin from one end of the cell to both ends. In the ssp1 mutant cells, cortical actin was localized only at one end, suggesting that the mutant fails to change growth polarity. Nucleotide sequence determination showed that ssp1+ encodes a novel protein kinase. Ectopic overexpression of ssp1+ resulted in an altered cell morphology and cortical actin was randomly dispersed within the cells. Immunocytological analysis revealed that the protein was primarily localized in the cytoplasm and that half of the protein existed in an insoluble fraction. These results show that the dynamics of actin-based growth polarity during the cell cycle are regulated, at least in part, by a novel set of protein kinases and phosphatases.
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