ArticleMolecular biology of the cell1995
Mutation or deletion of the Saccharomyces cerevisiae RAT3/NUP133 gene causes temperature-dependent nuclear accumulation of poly(A)+ RNA and constitutive clustering of nuclear pore complexes.
Article in Molecular biology of the cell, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers.
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Who cites it
61 citing papers in PubMed, 96 citations in OpenAlex.
- Distinct domains in Ndc1 mediate its interaction with the Nup84 complex and the nuclear membrane.The Journal of cell biology · 2023Article
- Mechanism of membrane-curvature generation by ER-tubule shaping proteins.Nature communications · 2021Article
- The yeast Ty1 retrotransposon requires components of the nuclear pore complex for transcription and genomic integration.Nucleic acids research · 2018Article
- Virtual Nuclear Envelope Breakdown and Its Regulators in Fission Yeast Meiosis.Frontiers in cell and developmental biology · 2016Review
- Review
- Analysis of the Lotus japonicus nuclear pore NUP107-160 subcomplex reveals pronounced structural plasticity and functional redundancy.Frontiers in plant science · 2013Article
- The yeast nuclear pore complex and transport through it.Genetics · 2012Article
- Structure-function mapping of a heptameric module in the nuclear pore complex.The Journal of cell biology · 2012Article
- A nucleoporin, Nup60p, affects the nuclear and cytoplasmic localization of ASH1 mRNA in S. cerevisiae.RNA (New York, N.Y.) · 2011Article
- Inner/Outer nuclear membrane fusion in nuclear pore assembly: biochemical demonstration and molecular analysis.Molecular biology of the cell · 2010Article
- Nuclear pore biogenesis into an intact nuclear envelope.Chromosoma · 2010Review
- Ty3 nuclear entry is initiated by viruslike particle docking on GLFG nucleoporins.Journal of virology · 2009Article
- Architectural nucleoporins Nup157/170 and Nup133 are structurally related and descend from a second ancestral element.The Journal of biological chemistry · 2009Article
- Transportin regulates major mitotic assembly events: from spindle to nuclear pore assembly.Molecular biology of the cell · 2009Article
- The structure of the scaffold nucleoporin Nup120 reveals a new and unexpected domain architecture.Structure (London, England : 1993) · 2009Article
- ER membrane-bending proteins are necessary for de novo nuclear pore formation.The Journal of cell biology · 2009Article
- Capture of AT-rich chromatin by ELYS recruits POM121 and NDC1 to initiate nuclear pore assembly.Molecular biology of the cell · 2008Article
- Centrin 2 localizes to the vertebrate nuclear pore and plays a role in mRNA and protein export.Molecular and cellular biology · 2008Article
- NUCLEOPORIN85 is required for calcium spiking, fungal and bacterial symbioses, and seed production in Lotus japonicus.The Plant cell · 2007Article
- The Nup107-160 nucleoporin complex is required for correct bipolar spindle assembly.Molecular biology of the cell · 2006Article
1 more citing papers are in PubMed but not listed here.
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7 authors at 1 institution in 1 country.
Funding
Abstract
To identify genes whose products play potential roles in the nucleocytoplasmic export of messenger RNA, we isolated temperature-sensitive strains of Saccharomyces cerevisiae and examined them by fluorescent in situ hybridization. With the use of a digoxigen-tagged oligo-(dT)50 probe, we identified those that showed nuclear accumulation of poly(A)+ RNA when cells were shifted to the nonpermissive temperature. We describe here the properties of yeast strains bearing the rat3-1 mutation (RAT-ribonucleic acid trafficking) and the cloning of the RAT3 gene. When cultured at the permissive temperature of 23 degrees C, fewer than 10% of cells carrying the rat3-1 allele showed nuclear accumulation of poly(A)+ RNA, whereas approximately 70% showed nuclear accumulation of poly(A)+ RNA, whereas approximately 70% showed nuclear accumulation of poly(A)+ RNA after a shift to 37 degrees C for 4 h. In wild-type cells, nuclear pore complexes (NPCs) are distributed relatively evenly around the nuclear envelope. Both indirect immunofluorescence analysis and electron microscopy of rat3-1 cells indicated that NPCs were clustered into one or a few regions of the NE in mutant cells. Similar NPC clustering was seen in mutant cells cultured at temperatures between 15 degrees C and 37 degrees C. The RAT3 gene encodes an 1157-amino acid protein without similarity to other known proteins. It is essential for growth only at 37 degrees C. Cells carrying a disruption of the RAT3 gene were very similar to cells carrying the original rat3-1 mutation; they showed temperature-dependent nuclear accumulation of poly(A)+ RNA and exhibited constitutive clustering of NPCs. Epitope tagging of Rat3p demonstrated that it is located at the nuclear periphery and co-localizes with nuclear pore proteins recognized by the RL1 monoclonal antibody. We refer to this nucleoporin as Rat3p/Nup133p.
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