ArticleProceedings of the National Academy of Sciences of the United States of America1995
Function of the p86 subunit of eukaryotic initiation factor (iso)4F as a microtubule-associated protein in plant cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 52 citations in OpenAlex.
- Plant Translation Factors and Virus Resistance.Viruses · 2015Review
- Purification and characterization of novel microtubule-associated proteins from Arabidopsis cell suspension cultures.Plant physiology · 2013Article
- YB-1 promotes microtubule assembly in vitro through interaction with tubulin and microtubules.BMC biochemistry · 2008Article
- The structure of eukaryotic translation initiation factor-4E from wheat reveals a novel disulfide bond.Plant physiology · 2007Article
- Tobacco mosaic virus movement protein functions as a structural microtubule-associated protein.Journal of virology · 2006Article
- Cap-independent translation of plant viral RNAs.Virus research · 2006Review
- Temporal and spatial expression patterns of TUB9, a beta-tubulin gene of Arabidopsis thaliana.Plant molecular biology · 2001Article
- The 65-kDa carrot microtubule-associated protein forms regularly arranged filamentous cross-bridges between microtubules.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Posttranscriptional control of gene expression in yeast.Microbiology and molecular biology reviews : MMBR · 1998Review
- Actin depolymerization affects stress-induced translational activity of potato tuber tissuePlant physiology · 1998Article
- Light-regulated changes in abundance and polyribosome association of ferredoxin mRNA are dependent on photosynthesis.The Plant cell · 1997Article
- The plant translational apparatus.Plant molecular biology · 1996Review
- End-to-end annealing of plant microtubules by the p86 subunit of eukaryotic initiation factor-(iso)4F.The Plant cell · 1995Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The isozyme form of eukaryotic initiation factor 4F [eIF-(iso)4F] from wheat germ is composed of a p28 subunit that binds the 7-methylguanine cap of mRNA and a p86 subunit having unknown function. The p86 subunit was found to have limited sequence similarity to a kinesin-like protein encoded by the katA gene of Arabidopsis thaliana. Native wheat germ eIF-(iso)4F and bacterially expressed p86 subunit and p86-p28 complex bound to taxol-stabilized maize microtubules (MTs) in vitro. Binding saturation occurred at 1 mol of p86 per 5-6 mol of polymerized tubulin dimer, demonstrating a substoichiometric interaction of p86 with MTs. No evidence was found for a direct interaction of the p28 subunit with MTs. Unlike kinesin, cosedimentation of eIF-(iso)4F with MTs was neither reduced by MgATP nor enhanced by adenosine 5'-[gamma-imido]triphosphate. Both p86 subunit and p86-p28 complex induced the bundling of MTs in vitro. The p86 subunit was immunolocalized to the cytosol in root maize cells and existed in three forms: fine particles, coarse particles, and linear patches. Many coarse particles and linear patches were colocalized or closely associated with cortical MT bundles in interphase cells. The results indicate that the p86 subunit of eIF-(iso)4F is a MT-associated protein that may simultaneously link the translational machinery to the cytoskeleton and regulate MT disposition in plant cells.
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