ArticleMolecular and cellular biology1995
A skeletal muscle-specific enhancer regulated by factors binding to E and CArG boxes is present in the promoter of the mouse myosin light-chain 1A gene.
Article in Molecular and cellular biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 91 citations in OpenAlex.
- Viral infection upregulates myostatin promoter activity in orange-spotted grouper (Epinephelus coioides).PloS one · 2017Article
- Comparative transcriptome analysis of muscular dystrophy models Large(myd), Dmd(mdx)/Large(myd) and Dmd(mdx): what makes them different?European journal of human genetics : EJHG · 2016Article
- Expression of the myosin heavy chain IIB gene in porcine skeletal muscle: the role of the CArG-Box promoter response element.PloS one · 2014Article
- SMYD1, the myogenic activator, is a direct target of serum response factor and myogenin.Nucleic acids research · 2009Article
- MicroRNA regulation of cardiovascular development.Circulation research · 2009Review
- Downregulation of SRF-FOS-JUNB pathway in fumarate hydratase deficiency and in uterine leiomyomas.Oncogene · 2009Article
- Myocyte-specific M-CAT and MEF-1 elements regulate G-protein gamma 3 gene (gamma3) expression in cardiac myocytes.DNA and cell biology · 2008Article
- Comparative in silico analysis identifies bona fide MyoD binding sites within the Myocyte stress 1 gene promoter.BMC molecular biology · 2008Article
- The myocardin-related transcription factor, MASTR, cooperates with MyoD to activate skeletal muscle gene expression.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Differentiation-dependent lysine 4 acetylation enhances MEF2C binding to DNA in skeletal muscle cells.Nucleic acids research · 2008Article
- Identification of a new hybrid serum response factor and myocyte enhancer factor 2-binding element in MyoD enhancer required for MyoD expression during myogenesis.Molecular biology of the cell · 2007Article
- Microphthalmia transcription factor isoforms in mast cells and the heart.Molecular and cellular biology · 2007Article
- A novel complex regulates cardiac actin gene expression through interaction of Emb, a class VI POU domain protein, MEF2D, and the histone transacetylase p300.Molecular and cellular biology · 2004Article
- Myosin light chain 1 atrial isoform (MLC1A) is expressed in pre-B cells under control of the BOB.1/OBF.1 coactivator.Nucleic acids research · 2004Article
- MyoD distal regulatory region contains an SRF binding CArG element required for MyoD expression in skeletal myoblasts and during muscle regeneration.Molecular biology of the cell · 2003Article
- Serum response factor plays an important role in the mechanically overloaded plantaris muscle of rats.Histochemistry and cell biology · 2003Article
- The myostatin gene is a downstream target gene of basic helix-loop-helix transcription factor MyoD.Molecular and cellular biology · 2002Article
- N-cadherin-dependent cell-cell contact regulates Rho GTPases and beta-catenin localization in mouse C2C12 myoblasts.The Journal of cell biology · 2002Article
- Serum response factor is crucial for actin cytoskeletal organization and focal adhesion assembly in embryonic stem cells.The Journal of cell biology · 2002Article
- Retinoid-dependent antagonism of serum response factor transactivation mediated by transcriptional coactivator proteins.Oncogene · 2001Article
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Authors and funding
6 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The mouse myosin light-chain 1A (MLC1A) gene, expressed in the atria of the adult heart, is one of the first muscle genes to be activated when skeletal as well as cardiac muscles form in the embryo. It is also transcribed in skeletal muscle cell lines at the onset of differentiation. Transient transfection assays of mouse skeletal muscle cell lines with DNA constructs containing MLC1A promoter fragments fused to the chloramphenicol acetyltransferase (CAT) gene show that the first 630 bp of the promoter is sufficient to direct expression of the reporter gene during myotube formation. Two E boxes located at bp -76 and -519 are necessary for this regulation. MyoD and myogenin proteins bind to them as heterodimers with E12 protein and, moreover, transactivate them in cotransfection experiments with the MLC1A promoter in nonmuscle cells. Interestingly, the effect of mutating each E box is less striking in primary cultures than in the C2 or Sol8 muscle cell line. A DNA fragment from bp -36 to -597 confers tissue- and stage-specific activity to the herpes simplex virus thymidine kinase promoter in both orientations, showing that the skeletal muscle-specific regulation of the MLC1A gene is under the control of a muscle-specific enhancer which extends into the proximal promoter region. At bp -89 is a diverged CArG box, CC(A/T)6AG, which binds the serum response factor (SRF) in myotube nuclear extracts, as does the wild-type sequence, CC(A/T)6GG. Both types of CArG box also bind a novel myotube-enriched complex which has contact points with the AT-rich part of the CArG box and adjacent 3' nucleotides. Mutations within the CArG box distinguish between the binding of this complex and binding of SRF; only SRF binding is directly involved in the specific regulation of the MLC1A gene in skeletal muscle cell lines.
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