ArticleMolecular and cellular biology1995
Mutational analysis of the Prt1 protein subunit of yeast translation initiation factor 3.
Article in Molecular and cellular biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- The Biological Roles of Translation Initiation Factor 3b.International journal of biological sciences · 2018Review
- Article
- Molecular mechanism of scanning and start codon selection in eukaryotes.Microbiology and molecular biology reviews : MMBR · 2011Review
- The C-terminal region of eukaryotic translation initiation factor 3a (eIF3a) promotes mRNA recruitment, scanning, and, together with eIF3j and the eIF3b RNA recognition motif, selection of AUG start codons.Molecular and cellular biology · 2010Article
- Processing bodies require RNA for assembly and contain nontranslating mRNAs.RNA (New York, N.Y.) · 2005Article
- Functional conservation of Dhh1p, a cytoplasmic DExD/H-box protein present in large complexes.Nucleic acids research · 2003Article
- Initiation-mediated mRNA decay in yeast affects heat-shock mRNAs, and works through decapping and 5'-to-3' hydrolysis.Nucleic acids research · 2003Article
- Yeast Lsm1p-7p/Pat1p deadenylation-dependent mRNA-decapping factors are required for brome mosaic virus genomic RNA translation.Molecular and cellular biology · 2003Article
- Direct eIF2-eIF3 contact in the multifactor complex is important for translation initiation in vivo.The EMBO journal · 2002Article
- Related eIF3 subunits TIF32 and HCR1 interact with an RNA recognition motif in PRT1 required for eIF3 integrity and ribosome binding.The EMBO journal · 2001Article
- Conidial germination in Aspergillus nidulans requires RAS signaling and protein synthesis.Genetics · 2000Article
- Mutations in translation initiation factors lead to increased rates of deadenylation and decapping of mRNAs in Saccharomyces cerevisiae.Molecular and cellular biology · 1999Article
- Upf1 and Upf2 proteins mediate normal yeast mRNA degradation when translation initiation is limited.Nucleic acids research · 1998Article
- Dbp5p/Rat8p is a yeast nuclear pore-associated DEAD-box protein essential for RNA export.The EMBO journal · 1998Article
- Identification of partners of TIF34, a component of the yeast eIF3 complex, required for cell proliferation and translation initiation.The EMBO journal · 1997Article
- Article
- Mutations in STS1 suppress the defect in 3' mRNA processing caused by the rna15-2 mutation in Saccharomyces cerevisiae.Molecular & general genetics : MGG · 1996Article
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6 authors.
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No grant is acknowledged in the PubMed record.
Abstract
The Saccharomyces cerevisiae PRT1 gene product Prt1p is a component of translation initiation factor eIF-3, and mutations in PRT1 inhibit translation initiation. We have investigated structural and functional aspects of Prt1p and its gene. Transcript analysis and deletion of the PRT1 5' end revealed that translation of PRT1 mRNA is probably initiated at the second in-frame ATG in the open reading frame. The amino acid changes encoded by six independent temperature-sensitive prt1 mutant alleles were found to be distributed throughout the central and C-terminal regions of Prt1p. The temperature sensitivity of each mutant allele was due to a single missense mutation, except for the prt1-2 allele, in which two missense mutations were required. In-frame deletion of an N-terminal region of Prt1p generated a novel, dominant-negative form of Prt1p that inhibits translation initiation even in the presence of wild-type Prt1p. Subcellular fractionation suggested that the dominant-negative Prt1p competes with wild-type Prt1p for association with a component of large Prt1p complexes and as a result inhibits the binding of wild-type Prt1p to the 40S ribosome.
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