ArticleMolecular and cellular biology1995
A 32-nucleotide exon-splicing enhancer regulates usage of competing 5' splice sites in a differential internal exon.
Article in Molecular and cellular biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
44 citing papers in PubMed, 92 citations in OpenAlex.
- Splicing of internal large exons is defined by novel cis-acting sequence elements.Nucleic acids research · 2012Article
- PLP/DM20 ratio is regulated by hnRNPH and F and a novel G-rich enhancer in oligodendrocytes.Nucleic acids research · 2007Article
- Identification of a splicing enhancer in MLH1 using COMPARE, a new assay for determination of relative RNA splicing efficiencies.Human molecular genetics · 2006Article
- Differential expression of splicing variants of the human caldesmon gene (CALD1) in glioma neovascularization versus normal brain microvasculature.The American journal of pathology · 2004Article
- An intronic enhancer regulates splicing of the twintron of Drosophila melanogaster prospero pre-mRNA by two different spliceosomes.Molecular and cellular biology · 2004Article
- Roles of hnRNP A1, SR proteins, and p68 helicase in c-H-ras alternative splicing regulation.Molecular and cellular biology · 2003Article
- SRp30c is a repressor of 3' splice site utilization.Molecular and cellular biology · 2002Article
- SR proteins and hnRNP H regulate the splicing of the HIV-1 tev-specific exon 6D.The EMBO journal · 2002Article
- Alternative splicing of U12-dependent introns in vivo responds to purine-rich enhancers.RNA (New York, N.Y.) · 2001Article
- Functionally antagonistic sequences are required for normal autoregulation of Drosophila tra-2 pre-mRNA splicing.Nucleic acids research · 2001Article
- Functional selection of splicing enhancers that stimulate trans-splicing in vitro.RNA (New York, N.Y.) · 2001Article
- Multiple features contribute to efficient constitutive splicing of an unusually large exon.Nucleic acids research · 2001Article
- A purine-rich intronic element enhances alternative splicing of thyroid hormone receptor mRNA.RNA (New York, N.Y.) · 2001Article
- Control of hnRNP A1 alternative splicing: an intron element represses use of the common 3' splice site.Molecular and cellular biology · 2000Article
- Splicing enhancement in the yeast rp51b intron.RNA (New York, N.Y.) · 2000Article
- AT-AC pre-mRNA splicing mechanisms and conservation of minor introns in voltage-gated ion channel genes.Molecular and cellular biology · 1999Review
- Modulation of exon skipping by high-affinity hnRNP A1-binding sites and by intron elements that repress splice site utilization.The EMBO journal · 1999Article
- Donor site competition is involved in the regulation of alternative splicing of the rat beta-tropomyosin pre-mRNA.RNA (New York, N.Y.) · 1999Article
- Expressional regulation of smooth muscle cell-specific genes in association with phenotypic modulation.Molecular and cellular biochemistry · 1999Review
- CA- and purine-rich elements form a novel bipartite exon enhancer which governs inclusion of the minute virus of mice NS2-specific exon in both singly and doubly spliced mRNAs.Molecular and cellular biology · 1999Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Large alternatively spliced internal exons are uncommon in vertebrate genes, and the mechanisms governing their usage are unknown. In this report, we examined alternative splicing of a 1-kb internal exon from the human caldesmon gene containing two regulated 5' splice sites that are 687 nucleotides apart. In cell lines normally splicing caldesmon RNA via utilization of the exon-internal 5' splice site, inclusion of the differential exon required a long purine-rich sequence located between the two competing 5' splice sites. This element consisted of four identical 32-nucleotide purine-rich repeats that resemble exon-splicing enhancers (ESE) identified in other genes. One 32-nucleotide repeat supported exon inclusion, repressed usage of the terminal 5' splice site, and functioned in a heterologous exon dependent on exon enhancers for inclusion, indicating that the caldesmon purine-rich sequence can be classified as an ESE. The ESE was required for utilization of the internal 5' splice site only in the presence of the competing 5' splice site and had no effect when placed downstream of the terminal 5' splice site. In the absence of the internal 5' splice site, the ESE activated a normally silent cryptic 5' splice site near the natural internal 5' splice site, indicating that the ESE stimulates upstream 5' splice site selection. We propose that the caldesmon ESE functions to regulate competition between two 5' splice sites within a differential internal exon.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.