ArticleThe Journal of physiology1995
Two distinct functional effects of protein phosphatase inhibitors on guinea-pig cardiac L-type Ca2+ channels.
Article in The Journal of physiology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 71 citations in OpenAlex.
- Downregulation of L-type CaHeart rhythm · 2026Article
- Mechanisms of the cyclic nucleotide cross-talk signaling network in cardiac L-type calcium channel regulation.Journal of molecular and cellular cardiology · 2017Article
- Pyruvate restores β-adrenergic sensitivity of L-type Ca(2+) channels in failing rat heart: role of protein phosphatase.American journal of physiology. Heart and circulatory physiology · 2013Article
- CaV1.2 signaling complexes in the heart.Journal of molecular and cellular cardiology · 2013Review
- Effect of okadaic acid on cultured clam heart cells: involvement of MAPkinase pathways.Biology open · 2012Article
- Targeting of protein phosphatases PP2A and PP2B to the C-terminus of the L-type calcium channel Ca v1.2.Biochemistry · 2010Article
- Colonic inflammation alters Src kinase-dependent gating properties of single Ca2+ channels via tyrosine nitration.American journal of physiology. Gastrointestinal and liver physiology · 2010Article
- Supramolecular assemblies and localized regulation of voltage-gated ion channels.Physiological reviews · 2009Review
- CaMKII-induced shift in modal gating explains L-type Ca(2+) current facilitation: a modeling study.Biophysical journal · 2009Article
- Pharmacological evidence for altered src kinase regulation of I (Ca,L) in patients with chronic atrial fibrillation.Naunyn-Schmiedeberg's archives of pharmacology · 2007Article
- Calcium channel function and regulation in beta 1- and beta 2-adrenoceptor transgenic mice.Naunyn-Schmiedeberg's archives of pharmacology · 2004Article
- Protein phosphatase 1 and an opposing protein kinase regulate steady-state L-type Ca2+ current in mouse cardiac myocytes.The Journal of physiology · 2004Article
- Regulation of L-type Ca2+ channels in the heart: overview of recent advances.Molecular and cellular biochemistry · 2003Review
- Modulation of cardiac Ca(V)1.2 channels by dihydropyridine and phosphatase inhibitor requires Ser-1142 in the domain III pore loop.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Novel functional properties of Ca(2+) channel beta subunits revealed by their expression in adult rat heart cells.The Journal of physiology · 2002Article
- Characterization of the cyclic nucleotide phosphodiesterase subtypes involved in the regulation of the L-type Ca2+ current in rat ventricular myocytes.British journal of pharmacology · 1999Article
- Functional characteristics of ES cell-derived cardiac precursor cells identified by tissue-specific expression of the green fluorescent protein.The Journal of cell biology · 1998Article
- Age-associated alterations in calcium current and its modulation in cardiac myocytes.Drugs & aging · 1998Review
- Effects of 2,3-butanedione monoxime (BDM) on calcium channels expressed in Xenopus oocytes.The Journal of physiology · 1998Article
- Generation of action potentials in a mathematical model of corticotrophs.Biophysical journal · 1997Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
1. The effects of the phosphatase inhibitors okadaic acid and calyculin A on single guinea-pig ventricular L-type Ca2+ channels were studied. The inactive derivative norokadaone was used as a negative control. 2. The two known effects of cAMP-dependent stimulation are mimicked by the phosphatase inhibitors to a varying extent. Only okadaic acid promotes the high-activity gating mode ('mode 2'), while calyculin A increases channel availability to a larger extent. As revealed by kinetic analysis of slow gating, the two phosphatase inhibitors retard a slow rate constant, which is assumed to represent exit from the available state by dephosphorylation. Norokadaone was inactive in both regards. 3. Mode 2 gating elicited by very positive prepulses is augmented by okadaic acid, and mode 2 lifetime is prolonged. Calyculin A fails to affect these parameters. Thus, voltage-facilitated mode 2 gating reveals the same pharmacological properties as the mode 2 sweeps observed using conventional pulse protocols. 4. The results are interpreted in terms of the different sensitivity of protein phosphatase subtypes towards the inhibitors: channel availability appears to be controlled by a phosphorylation site dephosphorylated by a type 1-like phosphatase, while mode 2 gating is coupled to a distinct site, dephosphorylated by a type 2A-like phosphatase.
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