ArticleThe Journal of cell biology1995
Analysis of MAP 4 function in living cells using green fluorescent protein (GFP) chimeras.
Article in The Journal of cell biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
61 citing papers in PubMed, 194 citations in OpenAlex.
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- Inhibition of cell expansion enhances cortical microtubule stability in the root apex of Arabidopsis thaliana.Journal of biological research (Thessalonike, Greece) · 2021Article
- Highly photostable fluorescent labeling of proteins in live cells using exchangeable coiled coils heterodimerization.Cellular and molecular life sciences : CMLS · 2020Article
- Microtubules and motor proteins support zebrafish neuronal migration by directing cargo.The Journal of cell biology · 2020Article
- HIV-associated neurodegeneration: exploitation of the neuronal cytoskeleton.Journal of neurovirology · 2019Review
- Measuring microtubule dynamics.Essays in biochemistry · 2018Review
- ISGylation - a key to lock the cell gates for preventing the spread of threats.Journal of cell science · 2017Review
- Microtubule-associated protein-4 controls nanovesicle dynamics and T cell activation.Journal of cell science · 2017Article
- The impact of mechanical compression on cortical microtubules in Arabidopsis: a quantitative pipeline.The Plant journal : for cell and molecular biology · 2016Article
- Techniques for studying protein trafficking and molecular motors in neurons.Cytoskeleton (Hoboken, N.J.) · 2016Review
- Xyloglucan Deficiency Disrupts Microtubule Stability and Cellulose Biosynthesis in Arabidopsis, Altering Cell Growth and Morphogenesis.Plant physiology · 2016Article
- Conserved Lysine Acetylation within the Microtubule-Binding Domain Regulates MAP2/Tau Family Members.PloS one · 2016Article
- P2X7 receptor activation regulates rapid unconventional export of transglutaminase-2.Journal of cell science · 2015Article
- Disruption of microtubules in plants suppresses macroautophagy and triggers starch excess-associated chloroplast autophagy.Autophagy · 2015Article
- The microtubule plus-end tracking proteins SPR1 and EB1b interact to maintain polar cell elongation and directional organ growth in Arabidopsis.The Plant cell · 2014Article
- FRIENDLY regulates mitochondrial distribution, fusion, and quality control in Arabidopsis.Plant physiology · 2014Article
- The role of microtubules and their dynamics in cell migration.The Journal of biological chemistry · 2012Article
- Structural evidence for cooperative microtubule stabilization by Taxol and the endogenous dynamics regulator MAP4.ACS chemical biology · 2012Article
- The Arabidopsis ARCP protein, CSI1, which is required for microtubule stability, is necessary for root and anther development.The Plant cell · 2012Article
- Systematic survey of deubiquitinase localization identifies USP21 as a regulator of centrosome- and microtubule-associated functions.Molecular biology of the cell · 2012Article
1 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
MAP 4 is a ubiquitous microtubule-associated protein thought to play a role in the polymerization and stability of microtubules in interphase and mitotic cells. We have analyzed the behavior of protein domains of MAP 4 in vivo using chimeras constructed from these polypeptides and the green fluorescent protein (GFP). GFP-MAP 4 localizes to microtubules; this is confirmed by colocalization of GFP-MAP 4 with microtubules that have incorporated microinjected rhodamine-tubulin, and by loss of localized fluorescence after treatment of cells with anti-microtubule agents. Different subdomains of MAP 4 have distinct effects on microtubule organization and dynamics. The entire basic domain of MAP 4 reorganizes microtubules into bundles and stabilizes these arrays against depolymerization with nocodazole. Within the basic domain, the PGGG repeats, which are conserved with MAP 2 and tau, have a weak affinity for microtubules and are dispensable for microtubule binding, whereas the MAP 4-unique PSP region can function independently in binding. The projection domain shows no microtubule localization, but does modulate the association of various binding subdomains with microtubules. The acidic carboxy terminus of MAP 4 strongly affects the microtubule binding characteristics of the other domains, despite constituting less than 6% of the protein. These data show that MAP 4 association with microtubules is modulated by sequences both within and outside the basic domain. Further, our work demonstrates that GFP chimeras will allow an in vivo analysis of the effects of MAPs and their variants on microtubule dynamics in real time.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.