ArticleThe Journal of cell biology1995
Expression of light meromyosin in Dictyostelium blocks normal myosin II function.
Article in The Journal of cell biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 24 citations in OpenAlex.
- A theoretical model of cytokinesis implicates feedback between membrane curvature and cytoskeletal organization in asymmetric cytokinetic furrowing.Molecular biology of the cell · 2016Article
- Characterization of three full-length human nonmuscle myosin II paralogs.The Journal of biological chemistry · 2013Article
- Nonmuscle myosin II is required for cell proliferation, cell sheet adhesion and wing hair morphology during wing morphogenesis.Developmental biology · 2010Article
- The effects of extracellular calcium on motility, pseudopod and uropod formation, chemotaxis, and the cortical localization of myosin II in Dictyostelium discoideum.Cell motility and the cytoskeleton · 2009Article
- Drosophila Myosin II, Zipper, is essential for ommatidial rotation.Developmental biology · 2007Article
- A novel guanine nucleotide exchange factor MyoGEF is required for cytokinesis.Cell cycle (Georgetown, Tex.) · 2006Article
- Localization of myosin II and V isoforms in cultured rat sympathetic neurones and their potential involvement in presynaptic function.The Journal of physiology · 2005Article
- Acto-myosin cytoskeleton dependent viscosity and shear-thinning behavior of the amoeba cytoplasm.European biophysics journal : EBJ · 2005Article
- Roles of cell adhesion and cytoskeleton activity in Entamoeba histolytica pathogenesis: a delicate balance.Infection and immunity · 2005Article
- Dictyostelium and Acanthamoeba myosin II assembly domains go to the cleavage furrow of Dictyostelium myosin II-null cells.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Involvement of an actomyosin contractile ring in Saccharomyces cerevisiae cytokinesis.The Journal of cell biology · 1998Article
- Virulence and functions of myosin II are inhibited by overexpression of light meromyosin in Entamoeba histolytica.Molecular biology of the cell · 1998Article
- Role of Dictyostelium racE in cytokinesis: mutational analysis and localization studies by use of green fluorescent protein.Molecular biology of the cell · 1997Article
- A novel member of the rho family of small GTP-binding proteins is specifically required for cytokinesis.The Journal of cell biology · 1996Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ability of myosin II to form filaments is essential for its function in vivo. This property of self association is localized in the light meromyosin (LMM) region of the myosin II molecules. To explore this property in more detail within the context of living cells, we expressed the LMM portion of the Dictyostelium myosin II heavy chain gene in wild-type Dictyostelium cells. We found that the LMM protein was expressed at high levels and that it folded properly into alpha-helical coiled-coiled molecules. The expressed LMM formed large cytoplasmic inclusions composed of entangled short filaments surrounded by networks of long tubular structures. Importantly, these abnormal structures sequestered the cell's native myosin II, completely removing it from its normal cytoplasmic distribution. As a result the cells expressing LMM displayed a myosin-null phenotype: they failed to undergo cytokinesis and became multinucleate, failed to form caps after treatment with Con A, and failed to complete their normal developmental cycle. Thus, expression of the LMM fragment in Dictyostelium completely abrogates myosin II function in vivo. The dominant-negative character of this phenotype holds promise as a general method to disrupt myosin II function in many cell types without the necessity of gene targeting.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.