ArticleInfection and immunity1995
A conserved Aeromonas salmonicida porin provides protective immunity to rainbow trout.
Article in Infection and immunity, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 95 citations in OpenAlex.
- Recombinant maltose-inducible porin LamB of Aeromonas hydrophila induces TMolecular biology reports · 2026Article
- Genomic Analysis ofPathogens (Basel, Switzerland) · 2024Article
- Effect of the Type VI Secretion System Secreted Protein Hcp on the Virulence ofMicroorganisms · 2022Article
- Effects of NaCl Concentration on the Behavior ofFoods (Basel, Switzerland) · 2022Article
- The challenge of efflux-mediated antibiotic resistance in Gram-negative bacteria.Clinical microbiology reviews · 2015Review
- The Aeromonas salmonicida subsp. salmonicida exoproteome: global analysis, moonlighting proteins and putative antigens for vaccination against furunculosis.Proteome science · 2013Article
- The major Aeromonas veronii outer membrane protein: gene cloning and sequence analysis.Current microbiology · 2005Article
- In vitro and in vivo studies of the Yrp1 protease from Yersinia ruckeri and its role in protective immunity against enteric red mouth disease of salmonids.Applied and environmental microbiology · 2003Article
- Simultaneous detection of Aeromonas salmonicida, Flavobacterium psychrophilum, and Yersinia ruckeri, three major fish pathogens, by multiplex PCR.Applied and environmental microbiology · 2002Article
- Colonic bacteria express an ulcerative colitis pANCA-related protein epitope.Infection and immunity · 2000Article
- The C-terminal domain of the Bordetella pertussis autotransporter BrkA forms a pore in lipid bilayer membranes.Journal of bacteriology · 1999Article
- Aeromonas salmonicida possesses two genes encoding homologs of the major outer membrane protein, OmpA.Journal of bacteriology · 1996Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A protein with an apparent M(r) of 28,000 was isolated from outer membrane preparations of Aeromonas salmonicida A440. The protein was tested for the ability to form pores, using a planar lipid bilayer model membrane system. The protein appeared to be a monomer with a single-channel conductance in 1.0 M KCl of 1.96 nS and a cation/anion permeability ratio of 2.91 +/- 0.68. These data show that the porin channel is comparable in size to OmpC and OmpF of Escherichia coli and is relatively nonselective, having some preference for cations over anions. The porin was purified by preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and a polyclonal antibody was raised. Immunoblot analysis showed that an immunologically cross-reactive protein was present in other Aeromonas strains but not in strains of Vibrio or Yersinia. The N-terminal amino acid sequence of the porin was determined and was found to show some homology to an Aeromonas hydrophila outer membrane protein. This is the second porin species of A. salmonicida to be described, and it differs from the other in subunit molecular weight, aggregation properties, peptidoglycan association, pore size, and antigenicity. Rainbow trout (Oncorhynchus mykiss) immunized intraperitoneally with the purified porin protein were significantly protected from experimental A. salmonicida challenge. This is the first report of successful vaccination against A. salmonicida with a purified outer membrane component.
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