Evidence map›Paper›PMID 7616968›Full record

ArticleMolecular & general genetics : MGG1995

A novel zinc finger protein encoded by a couch potato homologue from Solanum tuberosum enables a sucrose transport-deficient yeast strain to grow on sucrose.

C Kühn, W B Frommer

Abstract read
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In one paragraph

Article in Molecular & general genetics : MGG, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
3.9field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 21 citations in OpenAlex.

  1. Article
  2. Role of theInternational journal of molecular sciences · 2019
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

C KühnInstitut für Genbiologische Forschung, Berlin, Germany.
W B Frommer
Institut für biologische Forschung · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A yeast strain deficient in secreted invertase but expressing a cytoplasmic sucrose synthase has been used to select for potato genes that enable growth on sucrose as the sole carbon source by suppressing the sucrose uptake deficiency. Besides the already known sucrose transporter gene (StSUT1), ten different suppressor clones were identified and characterized. One of these cDNAs (PCP1) enabled efficient growth of the mutant yeast strain and mediated uptake of radiolabeled sucrose. The cDNA encodes a protein of 509 amino acids which is highly hydrophilic and thus does not seem to represent a transporter. Sequence comparisons show that the protein contains zinc finger motifs and shares weak homologies with the Drosophila couch potato gene, which serves as a transcriptional regulator, indicating that PCP1 activates a silent endogenous sucrose uptake system. The other suppressor clones encode either putative transcriptional regulators, protein kinases or enzymes involved in thiamine biosynthesis, ferredoxin reduction or glutamyl tRNA reduction and suppress the phenotype by unknown mechanisms.

Indexed as

Drosophila ProteinsMembrane Transport ProteinsAmino Acid SequenceBase SequenceBiological TransportCarrier ProteinsDNA, ComplementaryGene Expression Regulation, FungalGenes, SuppressorMolecular Sequence DataNuclear ProteinsPlant ProteinsSolanum tuberosumSucroseYeastsZinc FingersCarrier Proteinscpo protein, DrosophilaDNA, ComplementaryDrosophila ProteinsMembrane Transport ProteinsNuclear ProteinsPlant ProteinsSucrosesucrose transport protein, plant

Identifiers

PMID7616968
OpenAlexW2006802741

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.