Evidence map›Paper›PMID 7615742›Full record

ArticleJournal of clinical microbiology1995

Isolation and characterization of species-specific DNA probes from Taenia solium and Taenia saginata and their use in an egg detection assay.

A Chapman, V Vallejo, K G Mossie, D Ortiz, N Agabian, A Flisser

Open access · bronzeAbstract read
In one paragraph

Article in Journal of clinical microbiology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
1.0field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed, 64 citations in OpenAlex.

  1. Review
  2. Detection ofThe American journal of tropical medicine and hygiene · 2019
    Article
  3. Present status of laboratory diagnosis of human taeniosis/cysticercosis in Europe.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2017
    Review
  4. Article
  5. Molecular approaches to Taenia asiatica.The Korean journal of parasitology · 2013
    Review
  6. Article
  7. Article
  8. Article
  9. Immunoblot patterns of Taenia asiatica taeniasis.The Korean journal of parasitology · 2009
    Article
  10. Article
  11. Article
  12. Article
  13. Article
  14. Article
  15. Tapeworms.Current infectious disease reports · 2001
    Article
  16. Article
  17. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

A ChapmanDepartment of Pediatrics, University of California, San Francisco 94143, USA.
V Vallejo
K G Mossie
D Ortiz
N Agabian
A Flisser
University of California, San Francisco · US

Funding

REGULATION OF TRANSCRIPTION IN TRYPANOSOMESK11AI000986 · NIAID · UNIVERSITY OF CALIFORNIA SAN FRANCISCO · PI CHAPMAN, ALGER B · 1990 to 1994
–
NIAID NIH HHS K11 AI00986
6 · The paper itself

Abstract

Cysticercosis results from ingestion of the eggs of the tapeworm Taenia solium. Reduction of the incidence of human and swine cysticercosis requires identification and treatment of individuals who carry the adult tapeworm. T. solium and Taenia saginata eggs cannot be differentiated on the basis of morphology; thus, in order to improve existing methods for the diagnosis of taeniasis, we have developed highly sensitive, species-specific DNA probes which differentiate T. solium and T. saginata. Recombinant clones containing repetitive DNA sequences which hybridize specifically with genomic DNAs from either species were isolated and characterized. T. solium-specific DNA sequences contained complete and truncated forms of a tandemly repeated 158-bp DNA sequence. An unrelated T. saginata DNA sequence was also characterized and shown to encode a portion of the mitochondrial cytochrome c oxidase I gene. T. solium- and T. saginata-specific DNA probes did not hybridize in dot blot assays either with genomic DNA from the platyhelminths Taenia hydatigena, Taenia pisiformis, Taenia taeniaeformis, Echinococcus granulosus, and Schistosoma mansoni or with genomic DNA from other eukaryotes, including Saccharomyces cerevisiae, Candida albicans, Cryptosporidium parvum, Entamoeba histolytica, Trypanosoma gambiense, Trypanosoma brucei, and Giardia lamblia, Caenorhabditis elegans, and human DNA. By using these T. solium and T. saginata DNA probes, a rapid, highly sensitive and specific dot blot assay for the detection of T. solium eggs was developed.

Indexed as

AnimalsBase SequenceCloning, MolecularCysticercosisDNA, HelminthDNA ProbesDNA, RecombinantFemaleHumansMolecular Probe TechniquesMolecular Sequence DataOvumParasitologyRepetitive Sequences, Nucleic AcidRestriction MappingSensitivity and SpecificityDNA, HelminthDNA ProbesDNA, Recombinant

Identifiers

PMID7615742
PMCPMC228146
OpenAlexW2118720488

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.