ArticleThe Journal of cell biology1995
Actin-based movement of Listeria monocytogenes: actin assembly results from the local maintenance of uncapped filament barbed ends at the bacterium surface.
Article in The Journal of cell biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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Who cites it
52 citing papers in PubMed, 139 citations in OpenAlex.
- On the generation of force required for actin-based motility.Scientific reports · 2024Article
- Nascent adhesions shorten the period of lamellipodium protrusion through the Brownian ratchet mechanism.Molecular biology of the cell · 2023Article
- Recycling of the actin monomer pool limits the lifetime of network turnover.The EMBO journal · 2023Article
- Leading edge maintenance in migrating cells is an emergent property of branched actin network growth.eLife · 2022Article
- Morphological changes of plasma membrane and protein assembly during clathrin-mediated endocytosis.PLoS biology · 2018Article
- Global treadmilling coordinates actin turnover and controls the size of actin networks.Nature reviews. Molecular cell biology · 2017Review
- Arp2/3-mediated actin-based motility: a tail of pathogen abuse.Cell host & microbe · 2013Review
- Pathogens and polymers: microbe-host interactions illuminate the cytoskeleton.The Journal of cell biology · 2011Review
- Nephrin regulates lamellipodia formation by assembling a protein complex that includes Ship2, filamin and lamellipodin.PloS one · 2011Article
- Quantitative analysis of actin turnover in Listeria comet tails: evidence for catastrophic filament turnover.Biophysical journal · 2010Article
- Defining a core set of actin cytoskeletal proteins critical for actin-based motility of Rickettsia.Cell host & microbe · 2010Article
- Microfilament orientation constrains vesicle flow and spatial distribution in growing pollen tubes.Biophysical journal · 2009Article
- New proposed mechanism of actin-polymerization-driven motility.Biophysical journal · 2008Article
- Actin disassembly by cofilin, coronin, and Aip1 occurs in bursts and is inhibited by barbed-end cappers.The Journal of cell biology · 2008Article
- Diverse roles of actin in C. elegans early embryogenesis.BMC developmental biology · 2007Article
- Rapid actin monomer-insensitive depolymerization of Listeria actin comet tails by cofilin, coronin, and Aip1.The Journal of cell biology · 2006Article
- The yeast actin cytoskeleton: from cellular function to biochemical mechanism.Microbiology and molecular biology reviews : MMBR · 2006Review
- Mechanics and dynamics of actin-driven thin membrane protrusions.Biophysical journal · 2006Article
- Morphology of the lamellipodium and organization of actin filaments at the leading edge of crawling cells.Biophysical journal · 2005Article
- A plant-specific protein essential for blue-light-induced chloroplast movements.Plant physiology · 2005Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The thermodynamic basis for actin-based motility of Listeria monocytogenes has been investigated using cytoplasmic extracts of Xenopus eggs, initially developed by Theriot et al. (Theriot, J. A., J. Rosenblatt, D. A. Portnoy, P. J. Goldschmidt-Clermont, and T. J. Mitchison. 1994. Cell. 76:505-517) as an in vitro cell-free system. A large proportion (75%) of actin was found unpolymerized in the extracts. The amount of unassembled actin (12 microM) is accounted for by the sequestering functions of T beta 4Xen (20 microM) and profilin (5 microM), the barbed ends being capped. Movement of Listeria was not abolished by depletion of over 99% of the endogenous profilin. The proline-rich sequences of ActA are unlikely to be the target of profilin. All data support the view that actin assembly at the rear of Listeria results from a local shift in steady state due to a factor, keeping filaments uncapped, bound to the surface of the bacterium, while barbed ends are capped in the bulk cytoplasm. Movement is controlled by the energetic difference (i.e., the difference in critical concentration) between the two ends of the filaments, hence a constant ATP supply and the presence of barbed end capped F-actin in the medium are required to buffer free G-actin at a high concentration. The role of membrane components is demonstrated by the facts that: (a) Listeria movement can be reconstituted in the resuspended pellets of high speed-centrifuged extracts that are enriched in membranes; (b) Actin-based motility of endogenous vesicles, exhibiting the same rocketing movement as Listeria, can be observed in the extracts.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.