ArticleBiophysical journal1995
Calcium transients in cerebellar granule cell presynaptic terminals.
Article in Biophysical journal, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 81 papers.
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Who cites it
81 citing papers in PubMed, 166 citations in OpenAlex.
- Precise calcium-to-spike inference using biophysical generative models.bioRxiv : the preprint server for biology · 2026Article
- Monitoring presynaptic calcium dynamics with membrane-impermeant fluorescent indicators in motor nerve endings.Biophysical reviews · 2025Review
- Precise calcium-to-spike inference using biophysical generative models.Research square · 2025Article
- Mitochondrial phosphagen kinases support the volatile power demands of motor nerve terminals.The Journal of physiology · 2023Article
- Mitofusin 2 Sustains the Axonal Mitochondrial Network to Support Presynaptic CaThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2023Article
- Presynaptic PRRT2 Deficiency Causes Cerebellar Dysfunction and Paroxysmal Kinesigenic Dyskinesia.Neuroscience · 2020Article
- Mechanisms and Functional Consequences of Presynaptic Homeostatic Plasticity at Auditory Nerve Synapses.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2020Article
- GABA is a modulator, rather than a classical transmitter, in the medial nucleus of the trapezoid body-lateral superior olive sound localization circuit.The Journal of physiology · 2019Article
- Synaptotagmin 7 Mediates Both Facilitation and Asynchronous Release at Granule Cell Synapses.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2018Article
- Allosteric nanobodies uncover a role of hippocampal mGlu2 receptor homodimers in contextual fear consolidation.Nature communications · 2017Article
- A method for estimating intracellular ion concentration using optical nanosensors and ratiometric imaging.Scientific reports · 2017Article
- Dopamine Inhibition Differentially Controls Excitability of Substantia Nigra Dopamine Neuron Subpopulations through T-Type Calcium Channels.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2017Article
- N- and L-Type Voltage-Gated Calcium Channels Mediate Fast Calcium Transients in Axonal Shafts of Mouse Peripheral Nerve.Frontiers in cellular neuroscience · 2016Article
- Estimation of presynaptic calcium currents and endogenous calcium buffers at the frog neuromuscular junction with two different calcium fluorescent dyes.Frontiers in synaptic neuroscience · 2014Article
- Differential triggering of spontaneous glutamate release by P/Q-, N- and R-type Ca2+ channels.Nature neuroscience · 2013Article
- Visualization of synaptic inhibition with an optogenetic sensor developed by cell-free protein engineering automation.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- Mitochondria and plasma membrane Ca2+-ATPase control presynaptic Ca2+ clearance in capsaicin-sensitive rat sensory neurons.The Journal of physiology · 2013Article
- Postnatal loss of P/Q-type channels confined to rhombic-lip-derived neurons alters synaptic transmission at the parallel fiber to purkinje cell synapse and replicates genomic Cacna1a mutation phenotype of ataxia and seizures in mice.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- Modulation by pregnenolone sulfate of filtering properties in the hippocampal trisynaptic circuit.Hippocampus · 2012Article
- Functional integration of calcium regulatory mechanisms at Purkinje neuron synapses.Cerebellum (London, England) · 2012Review
21 more citing papers are in PubMed but not listed here.
Corrections and comments
- Erratum issued
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Calcium ions act presynaptically to modulate synaptic strength and to trigger neurotransmitter release. Here we detect stimulus-evoked changes in residual free calcium ([Ca2+]i) in rat cerebellar granule cell presynaptic terminals. Granule cell axons, known as parallel fibers, and their associated boutons, were labeled with several calcium indicators. When parallel fibers were extracellularly activated with stimulus trains, calcium accumulated in the terminals, producing changes in the fluorescence of the indicators. During the stimulus train, the fluorescence change per pulse became progressively smaller with the high affinity indicators Fura-2 and calcium green-2 but remained constant with the low affinity dyes BTC and furaptra. In addition, fluorescence transients of high affinity dyes were slower than those of low affinity indicators, which appear to accurately report the time course of calcium transients. Simulations show that differences in the observed transients can be explained by the different affinities and off rates of the fluorophores. The return of [Ca2+]i to resting levels can be approximated by an exponential decay with a time constant of 150 ms. On the basis of the degree of saturation in the response of high affinity dyes observed during trains, we estimate that each action potential increases [Ca2+]i in the terminal by several hundred nanomolar. These findings indicate that in these terminals [Ca2+]i transients are much larger and faster than those observed in larger boutons, such as those at the neuromuscular junction. Such rapid [Ca2+]i dynamics may be found in many of the terminals in the mammalian brain that are similar in size to parallel fiber boutons.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.