ArticleBiophysical journal1995
Effect of concentration quenching on fluorescence recovery after photobleaching measurements.
Article in Biophysical journal, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 61 citations in OpenAlex.
- Synthetic biomolecular condensates enhance translation from a target mRNA in living cells.Nature chemistry · 2025Article
- Ultrafast Collective Excited-State Dynamics of a Virus-Supported Fluorophore Antenna.The journal of physical chemistry letters · 2022Article
- Nanoscale Functionalized Particles with Rotation-Controlled Capture in Shear Flow.ACS applied materials & interfaces · 2018Article
- Fouling-Resistant Hydrogels Prepared by the Swelling-Assisted Infusion and Polymerization of Dopamine.ACS applied bio materials · 2018Article
- A bottom-up approach to understanding protein layer formation at solid-liquid interfaces.Advances in colloid and interface science · 2014Review
- Spectroscopic study of porphyrin-caffeine interactions.Journal of fluorescence · 2012Article
- A comparison of fluorescent stains for the assessment of viability and metabolic activity of lactic acid bacteria.World journal of microbiology & biotechnology · 2012Article
- Laterally mobile, functionalized self-assembled monolayers at the fluorous-aqueous interface in a plug-based microfluidic system: characterization and testing with membrane protein crystallization.Journal of the American Chemical Society · 2009Article
- Fluorescence recovery after photobleaching: a versatile tool for mobility and interaction measurements in pharmaceutical research.Pharmaceutical research · 1999Review
- Diffusion measurement of fluorescence-labeled amphiphilic molecules with a standard fluorescence microscope.Biophysical journal · 1997Article
- Influence of the triplet excited state on the photobleaching kinetics of fluorescein in microscopy.Biophysical journal · 1996Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Standard analysis of fluorescence recovery after photobleaching (FRAP) data is valid only if the quantum yield of unphotobleached fluorophores is independent of concentration, yet close molecular packing in two-dimensional systems may lead to significant fluorescence concentration quenching. Using total internal reflection fluorescence, we quantified the surface concentration dependence of the relative quantum yield of fluorescein isothiocyanate-labeled proteins adsorbed to polymeric surfaces before performing measurements of fluorescence recovery after pattern photobleaching. Adsorbed layers of FITC-labeled ribonuclease A displayed significant concentration quenching, and thus the standard FRAP analysis method was unacceptable. We present an extended FRAP analysis procedure that accounts for the changing quantum yield of diffusing fluorophores in systems that are influenced by concentration quenching. The extended analysis shows that if concentration quenching conditions prevail, there may be significant error in the transport parameters obtained from FRAP measurements by using the standard procedures.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.