ArticleBiophysical journal1995
Mechanically stimulated cytoskeleton rearrangement and cortical contraction in human neutrophils.
Article in Biophysical journal, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 46 citations in OpenAlex.
- Fluid Shear Stress Enhances the Phagocytic Response of Astrocytes.Frontiers in bioengineering and biotechnology · 2020Article
- Modeling the Mechanosensitivity of Neutrophils Passing through a Narrow Channel.Biophysical journal · 2015Article
- Measuring forces at the leading edge: a force assay for cell motility.Integrative biology : quantitative biosciences from nano to macro · 2013Article
- Increasing membrane tension decreases miscibility temperatures; an experimental demonstration via micropipette aspiration.Biophysical journal · 2012Article
- Cell membrane tethers generate mechanical force in response to electrical stimulation.Biophysical journal · 2010Article
- Micromechanics of filopodia mediated capture of pathogens by macrophages.European biophysics journal : EBJ · 2007Article
- Prestressed F-actin networks cross-linked by hinged filamins replicate mechanical properties of cells.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Multiple membrane tethers probed by atomic force microscopy.Biophysical journal · 2005Article
- FRET measurements of cell-traction forces and nano-scale clustering of adhesion ligands varied by substrate stiffness.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Microviscoelasticity of the apical cell surface of human umbilical vein endothelial cells (HUVEC) within confluent monolayers.Biophysical journal · 2004Article
- Membrane tethers formed from blood cells with available area and determination of their adhesion energy.Biophysical journal · 2002Article
- Myosin I contributes to the generation of resting cortical tension.Biophysical journal · 1999Article
- Hydrodynamics of micropipette aspiration.Biophysical journal · 1999Article
- Use of green fluorescent protein-conjugated beta-actin as a novel molecular marker for in vitro tumor cell chemotaxis assay.Biotechnology progressArticle
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
A mechanical test with micropipets is used to characterize cytoskeleton rearrangement and contraction induced by mechanical stresses in human neutrophils. The yield shear resultant of the cell cortex is on the order of 0.06 to 0.09 mN.m-1. The measured yield shear resultant suggests that the neutrophil cortex is a weakly cross-linked structure. When a tether is pulled out from the cell surface, a polymer structure starts to fill it and spreads out from the cell body. The rate of advancement of the polymerization front is almost constant and, therefore, is not diffusion limited. The measured rate is much smaller than the one of spontaneous actin polymerization, suggesting that the limiting process is either the dissociation of actin monomers from their dimers with the capping proteins or the rate of formation of new nucleation sites or both. Polymerization is also observed after applying sufficient mechanical stresses on a small portion of the cell surface. The polymerization is followed by mass transfer from the cell into the prestressed region and later on by contraction of the main cell body. The pressure generating the flow is located in the prestressed region and most probably is a result of its "swelling" and contraction. The contraction of the main cell body is very similar (in its time dependence and magnitude) to the contraction during phagocytosis. The measured maximum cortical tension is on the order of 0.5 mN.m-1, which for a 3.5-microns diameter pipet corresponds to a maximum contraction force of 11 nN.
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Registered trials
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