ArticleBiophysical journal1995
Millisecond measurement of transport during and after an electroporation pulse.
Article in Biophysical journal, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 69 citations in OpenAlex.
- Optimizing electroporation via pulse modulation: a molecular dynamics study.European biophysics journal : EBJ · 2025Article
- Recent Advances in Microscale Electroporation.Chemical reviews · 2022Review
- How physical techniques improve the transdermal permeation of therapeutics: A review.Medicine · 2022Review
- Pulse Duration Dependent Asymmetry in Molecular Transmembrane Transport Due to Electroporation in H9c2 Rat Cardiac Myoblast Cells In Vitro.Molecules (Basel, Switzerland) · 2021Article
- Intracellular Delivery by Membrane Disruption: Mechanisms, Strategies, and Concepts.Chemical reviews · 2018Review
- Calcein Release from Cells In Vitro via Reversible and Irreversible Electroporation.The Journal of membrane biology · 2018Article
- Mechanisms of transfer of bioactive molecules through the cell membrane by electroporation.European biophysics journal : EBJ · 2015Review
- Evidence for electro-induced membrane defects assessed by lateral mobility measurement of a GPi anchored protein.European biophysics journal : EBJ · 2014Article
- Scaling relationship and optimization of double-pulse electroporation.Biophysical journal · 2014Article
- Physical non-viral gene delivery methods for tissue engineering.Annals of biomedical engineering · 2013Review
- Second Harmonic Generation for time-resolved monitoring of membrane pore dynamics subserving electroporation of neurons.Biomedical optics express · 2011Article
- Membrane binding of plasmid DNA and endocytic pathways are involved in electrotransfection of mammalian cells.PloS one · 2011Article
- Kinetics of transmembrane transport of small molecules into electropermeabilized cells.Biophysical journal · 2008Article
- Numerical calculations of single-cell electroporation with an electrolyte-filled capillary.Biophysical journal · 2007Article
- Simultaneous maximization of cell permeabilization and viability in single-cell electroporation using an electrolyte-filled capillary.Analytical chemistry · 2007Article
- Electroosmotic enhancement of the binding of a neutral molecule to a transmembrane pore.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- The effects of intense submicrosecond electrical pulses on cells.Biophysical journal · 2003Article
- Time courses of mammalian cell electropermeabilization observed by millisecond imaging of membrane property changes during the pulse.Biophysical journal · 1999Article
- Direct observation in the millisecond time range of fluorescent molecule asymmetrical interaction with the electropermeabilized cell membrane.Biophysical journal · 1997Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Electroporation involves the application of an electric field pulse that creates transient aqueous pathways in lipid bilayer membranes. Transport through these pathways can occur by different mechanisms during and after a pulse. To determine the time scale of transport and the mechanism(s) by which it occurs, efflux of a fluorescent molecule, calcein, across erythrocyte ghost membranes was measured with a fluorescence microscope photometer with millisecond time resolution during and after electroporation pulses several milliseconds in duration. One of four outcomes was typically observed. Ghosts were: (1) partially emptied of calcein, involving efflux primarily after the pulse; (2) completely emptied of calcein, involving efflux primarily after the pulse; (3) completely emptied of calcein, involving efflux both during and after the pulse; or (4) completely emptied of calcein, involving efflux primarily during the pulse. Partial emptying, involving significant efflux during the pulse, was generally not observed. We conclude that under some conditions transport caused by electroporation occurs predominantly by electrophoresis and/or electroosmosis during a pulse, although under other conditions transport occurs in part or almost completely by diffusion within milliseconds to seconds after a pulse.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.