ArticleNucleic acids research1995
Functional domains of the heavy metal-responsive transcription regulator MTF-1.
Article in Nucleic acids research, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 155 citations in OpenAlex.
- Emerging perspectives of copper-mediated transcriptional regulation in mammalian cell development.Metallomics : integrated biometal science · 2024Review
- The LUFS domain, its transcriptional regulator proteins, and drug resistance in the fungal pathogen Candida auris.Protein science : a publication of the Protein Society · 2019Article
- A T > G Mutation in theFrontiers in genetics · 2019Article
- Short-lived mammals (shrew, mouse) have a less robust metal-responsive transcription factor than humans and bats.Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine · 2016Article
- The zinc finger protein ZNF658 regulates the transcription of genes involved in zinc homeostasis and affects ribosome biogenesis through the zinc transcriptional regulatory element.Molecular and cellular biology · 2015Article
- Inhibition of endogenous MTF-1 signaling in zebrafish embryos identifies novel roles for MTF-1 in development.Biochimica et biophysica acta · 2014Article
- Efficient metal-specific transcription activation by Drosophila MTF-1 requires conserved cysteine residues in the carboxy-terminal domain.Biochimica et biophysica acta · 2012Article
- The role of small ubiquitin-like modifier-interacting motif in the assembly and regulation of metal-responsive transcription factor 1.The Journal of biological chemistry · 2011Article
- Hypoxia acts through multiple signaling pathways to induce metallothionein transactivation by the metal-responsive transcription factor-1 (MTF-1).Biochemistry and cell biology = Biochimie et biologie cellulaire · 2011Article
- Nematode and snail metallothioneins.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2011Review
- Characterization of MtnE, the fifth metallothionein member in Drosophila.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2011Article
- Roles of two activation domains in Zap1 in the response to zinc deficiency in Saccharomyces cerevisiae.The Journal of biological chemistry · 2011Article
- Metal transcription factor-1 regulation via MREs in the transcribed regions of selenoprotein H and other metal-responsive genes.Biochimica et biophysica acta · 2010Article
- Metal-responsive transcription factor 1 (MTF-1) activity is regulated by a nonconventional nuclear localization signal and a metal-responsive transactivation domain.Molecular and cellular biology · 2009Article
- Nickel mobilizes intracellular zinc to induce metallothionein in human airway epithelial cells.American journal of respiratory cell and molecular biology · 2009Article
- Induction of metallothionein I by arsenic via metal-activated transcription factor 1: critical role of C-terminal cysteine residues in arsenic sensing.The Journal of biological chemistry · 2009Article
- STAT5 proteins are involved in down-regulation of iron regulatory protein 1 gene expression by nitric oxide.The Biochemical journal · 2006Article
- The zinc-sensing mechanism of mouse MTF-1 involves linker peptides between the zinc fingers.Molecular and cellular biology · 2006Article
- Radiation carcinogenesis in mouse thymic lymphomas.Cancer science · 2006Review
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Metallothioneins (MTs) constitute a class of low molecular weight, cysteine-rich, metal binding proteins which are regulated at the level of gene transcription in response to heavy metals and other adverse treatments. We have previously cloned a zinc finger factor (MTF-1) that binds specifically to heavy metal-responsive DNA sequence elements in metallothionein promoters and shown that this factor is essential for basal and heavy metal-induced transcription. Here we report that the C-terminal part of MTF-1 downstream of the DNA binding zinc fingers harbours three different transactivation domains, namely an acidic domain, a proline-rich domain and a domain rich in serine and threonine. When fused to the heterologous DNA binding domain of the yeast factor GAL4 these activation domains function constitutively, i.e. transcription of a GAL4-driven reporter gene is not induced by heavy metals. In search of the region(s) responsible for metal induction, external and internal deletion mutations of mouse and human MTF-1 and chimeric variants thereof were tested with a reporter gene driven by a metal-responsive promoter. The N-terminal part of MTF-1 containing the zinc fingers, which are dependent on zinc for efficient DNA binding, can indeed confer a limited (3- to 4-fold) zinc-responsive transcription when fused to the heterologous activation domain of the viral VP16 protein. Another region containing the acidic and proline-rich activation domains also contributes to metal inducibility, but only in the context of intact MTF-1. This indicates that the activity of MTF-1 results from a complex interplay of different functional domains.
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