ArticleNucleic acids research1995
Autoregulation of poly(A)-binding protein synthesis in vitro.
Article in Nucleic acids research, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
49 citing papers in PubMed, 112 citations in OpenAlex.
- Proteolytic dissection of eIF4G reveals the closed-loop mRNP as an architecture for translation repression.bioRxiv : the preprint server for biology · 2026Article
- HDX-MS finds that partial unfolding with sequential domain activation controls condensation of a cellular stress marker.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Roles of mRNA poly(A) tails in regulation of eukaryotic gene expression.Nature reviews. Molecular cell biology · 2022Review
- The role of CSDE1 in translational reprogramming and human diseases.Cell communication and signaling : CCS · 2020Review
- eIF4G2 balances its own mRNA translation via a PCBP2-based feedback loop.RNA (New York, N.Y.) · 2019Article
- Translation Control ofInternational journal of molecular sciences · 2019Review
- Article
- Sbp1 modulates the translation of Pab1 mRNA in a poly(A)- and RGG-dependent manner.RNA (New York, N.Y.) · 2018Article
- Autogenous cross-regulation ofGenes & development · 2017Article
- Article
- Eukaryotic translation initiation factor 4G (eIF4G) coordinates interactions with eIF4A, eIF4B, and eIF4E in binding and translation of the barley yellow dwarf virus 3' cap-independent translation element (BTE).The Journal of biological chemistry · 2017Article
- The organization and regulation of mRNA-protein complexes.Wiley interdisciplinary reviews. RNA · 2017Review
- Pervasive isoform-specific translational regulation via alternative transcription start sites in mammals.Molecular systems biology · 2016Article
- Cytoplasmic poly(A) binding protein-1 binds to genomically encoded sequences within mammalian mRNAs.RNA (New York, N.Y.) · 2016Article
- Biochemical identification of new proteins involved in splicing repression at the Drosophila P-element exonic splicing silencer.Genes & development · 2015Article
- A posttranscriptional mechanism that controls Ptbp1 abundance in the Xenopus epidermis.Molecular and cellular biology · 2015Article
- Probing the closed-loop model of mRNA translation in living cells.RNA biology · 2015Article
- mRNA translation and protein synthesis: an analysis of different modelling methodologies and a new PBN based approach.BMC systems biology · 2014Article
- Posttranscriptional regulation of insulin family ligands and receptors.International journal of molecular sciences · 2013Review
- Rotavirus prevents the expression of host responses by blocking the nucleocytoplasmic transport of polyadenylated mRNAs.Journal of virology · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The poly(A)-binding protein (PABP), in a complex with the 3'poly(A) tail of eukaryotic mRNAs, plays important roles in the control of translation and message stability. All known examples of PABP mRNAs contain an extensive A-rich sequence in their 5' untranslated regions. Studies in mammalian cells undergoing growth stimulation or terminal differentiation indicate that PABP expression is regulated at the translational level. Here we examine the hypothesis that synthesis of the PABP is autogenously controlled. We show that the endogenous inactive PABP mRNA in rabbit reticulocytes can be specifically stimulated by addition of low concentrations of poly(A) and that this stimulation is also observed with in vitro transcribed human PABP mRNA. By deleting the A-rich region from the leader of human PABP mRNA and adding it upstream of the initiator AUG in a reporter mRNA we show that the adenylate tract is sufficient and necessary for mRNA repression and poly(A)-mediated activation in the reticulocyte cell-free system. UV cross-linking experiments demonstrate that the leader adenylate tract binds PABP. Furthermore, addition of recombinant GST-PABP to the cell-free system represses translation of mRNAs containing the A-rich sequence in their 5'UTR, but has no effect on control mRNA. We thus conclude that in vitro PABP binding to the A-rich sequence in the 5' UTR of PABP mRNA represses its own synthesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.