ArticleJournal of virology1995
Substrate specificity of Ty1 integrase.
Article in Journal of virology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 30 citations in OpenAlex.
- Ty1 integrase is composed of an active N-terminal domain and a large disordered C-terminal module dispensable for its activity in vitro.The Journal of biological chemistry · 2021Article
- Ty1 integrase overexpression leads to integration of non-Ty1 DNA fragments into the genome of Saccharomyces cerevisiae.Molecular genetics and genomics : MGG · 2010Review
- Identification and characterization of critical cis-acting sequences within the yeast Ty1 retrotransposon.RNA (New York, N.Y.) · 2005Article
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- Article
- DNA integration by Ty integrase in yku70 mutant Saccharomyces cerevisiae cells.Molecular and cellular biology · 2000Article
- Correct integration of model substrates by Ty1 integrase.Journal of virology · 2000Article
- Involvement of DNA end-binding protein Ku in Ty element retrotransposition.Molecular and cellular biology · 1999Article
- Reverse transcription of a self-primed retrotransposon requires an RNA structure similar to the U5-IR stem-loop of retroviruses.Molecular and cellular biology · 1998Article
- Insertion sequences.Microbiology and molecular biology reviews : MMBR · 1998Review
- In vivo Ty1 reverse transcription can generate replication intermediates with untidy ends.Journal of virology · 1998Article
- The application of a homologous recombination assay revealed amino acid residues in an LTR-retrotransposon that were critical for integration.Journal of virology · 1998Article
- Plus-strand strong-stop DNA transfer in yeast Ty retrotransposons.The EMBO journal · 1997Article
- pCal, a highly unusual Ty1/copia retrotransposon from the pathogenic yeast Candida albicans.Journal of bacteriology · 1997Article
- Retrovirus-like end processing of the tobacco Tnt1 retrotransposon linear intermediates of replication.Journal of virology · 1997Article
- Increased length of long terminal repeats inhibits Ty1 transposition and leads to the formation of tandem multimers.Genetics · 1997Article
- Influence of subterminal viral DNA nucleotides on differential susceptibility to cleavage by human immunodeficiency virus type 1 and visna virus integrases.Journal of virology · 1996Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Integration of the Saccharomyces cerevisiae retrotransposon Ty1 requires the element-encoded integrase (IN) protein, which is a component of cytoplasmic virus-like particles (VLPs). Using purified recombinant Ty1 IN and an oligonucleotide integration assay based on Ty1 long terminal repeat sequences, we have compared IN activity on substrates having either wild-type or altered donor ends. IN showed a marked preference for blunt-end substrates terminating in an A:T pair over substrates ending in a G:C pair or a 3' dideoxyadenosine. VLP activity on representative substrates also showed preference for donor strands which have an adenosine terminus. Staggered-end substrates showed little activity when nucleotides were removed from the end of the wild-type donor strand, but removal of one nucleotide from the complementary strand did not significantly diminish activity. Removal of additional nucleotides from the complementary strand reduced activity to minimal detection levels. These results suggest that the sequence specificity of Ty1 IN is not stringent in vitro. The absence of Ty1 IN-mediated 3' dinucleotide cleavage, a characteristic of retroviral integrases, was demonstrated by using selected substrates. In addition to the forward reaction, both recombinant IN and VLP-associated IN carry out the reverse disintegration reaction with long terminal repeat-based dumbbell substrates. Disintegration activity exhibits sequence preferences similar to those observed for the forward reaction.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.