ArticleProceedings of the National Academy of Sciences of the United States of America1995
Dissection of transcription factor TFIIF functional domains required for initiation and elongation.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 79 citations in OpenAlex.
- From chromatin to proteostasis: multilevel gene regulation in Mendelian diseases.Frontiers in epigenetics and epigenomics · 2026Review
- Mechanism of mammalian transcriptional repression by noncoding RNA.Nature structural & molecular biology · 2025Article
- The long transcript of lncRNA TMPO-AS1 promotes bone metastases of prostate cancer by regulating the CSNK2A1/DDX3X complex in Wnt/β-catenin signaling.Cell death discovery · 2023Article
- Transcriptome sequencing and metabolome analysis of food habits domestication from live prey fish to artificial diets in mandarin fish (Siniperca chuatsi).BMC genomics · 2021Article
- Regulation of hepatocyte cell cycle re-entry by RNA polymerase II-associated Gdown1.Cell cycle (Georgetown, Tex.) · 2020Review
- Architecture of Pol II(G) and molecular mechanism of transcription regulation by Gdown1.Nature structural & molecular biology · 2018Article
- Born to run: control of transcription elongation by RNA polymerase II.Nature reviews. Molecular cell biology · 2018Review
- RNA polymerase III-specific general transcription factor IIIC contains a heterodimer resembling TFIIF Rap30/Rap74.Nucleic acids research · 2013Article
- Control of transcriptional elongation.Annual review of genetics · 2013Review
- Identification of Tat-SF1 cellular targets by exon array analysis reveals dual roles in transcription and splicing.RNA (New York, N.Y.) · 2011Article
- Position of the general transcription factor TFIIF within the RNA polymerase II transcription preinitiation complex.The EMBO journal · 2010Article
- Architecture of the RNA polymerase II-TFIIF complex revealed by cross-linking and mass spectrometry.The EMBO journal · 2010Article
- Phosphorylation of serine 177 of the small hepatitis delta antigen regulates viral antigenomic RNA replication by interacting with the processive RNA polymerase II.Journal of virology · 2010Article
- Kap104p imports the PY-NLS-containing transcription factor Tfg2p into the nucleus.The Journal of biological chemistry · 2009Article
- Human RNA polymerase II elongation in slow motion: role of the TFIIF RAP74 alpha1 helix in nucleoside triphosphate-driven translocation.Molecular and cellular biology · 2005Article
- In vivo evidence that defects in the transcriptional elongation factors RPB2, TFIIS, and SPT5 enhance upstream poly(A) site utilization.Molecular and cellular biology · 2003Article
- FCP1, a phosphatase specific for the heptapeptide repeat of the largest subunit of RNA polymerase II, stimulates transcription elongation.Molecular and cellular biology · 2002Article
- Genetic evidence supports a role for the yeast CCR4-NOT complex in transcriptional elongation.Genetics · 2001Article
- TFIIH action in transcription initiation and promoter escape requires distinct regions of downstream promoter DNA.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- Tat-SF1 protein associates with RAP30 and human SPT5 proteins.Molecular and cellular biology · 1999Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
TFIIF is unique among the general transcription factors because of its ability to control the activity of RNA polymerase II at both the initiation and elongation stages of transcription. Mammalian TFIIF, a heterodimer of approximately 30-kDa (RAP30) and approximately 70-kDa (RAP74) subunits, assists TFIIB in recruiting RNA polymerase II into the preinitiation complex and activates the overall rate of RNA chain elongation by suppressing transient pausing by polymerase at many sites on DNA templates. A major objective of efforts to understand how TFIIF regulates transcription has been to establish the relationship between its initiation and elongation activities. Here we establish this relationship by demonstrating that TFIIF transcriptional activities are mediated by separable functional domains. To accomplish this, we sought and identified distinct classes of RAP30 mutations that selectively block TFIIF activity in transcription initiation and elongation. We propose that (i) TFIIF initiation activity is mediated at least in part by RAP30 C-terminal sequences that include a cryptic DNA-binding domain similar to conserved region 4 of bacterial sigma factors and (ii) TFIIF elongation activity is mediated in part by RAP30 sequences located immediately upstream of the C terminus in a region proposed to bind RNA polymerase II and by additional sequences located in the RAP30 N terminus.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.