ArticleNucleic acids research1995
Effect of competing self-structure on triplex formation with purine-rich oligodeoxynucleotides containing GA repeats.
Article in Nucleic acids research, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 71 citations in OpenAlex.
- The Influence of CG sites on dynamic DNA sequence mutagenesis in the genomic evolution of mammalian lifespan.Nucleic acids research · 2025Article
- Article
- In vitro selection of oligonucleotides that bind double-stranded DNA in the presence of triplex-stabilizing agents.Nucleic acids research · 2010Article
- Evaluation of effects of bivalent cations on the formation of purine-rich triple-helix DNA by ESI-FT-MS.Journal of the American Society for Mass Spectrometry · 2009Article
- Sequence-specific triple helix formation with genomic DNA.Biochemistry · 2007Article
- Bioconjugation of oligonucleotides for treating liver fibrosis.Oligonucleotides · 2007Review
- Peptide conjugates for chromosomal gene targeting by triplex-forming oligonucleotides.Nucleic acids research · 2004Article
- Design of a novel triple helix-forming oligodeoxyribonucleotide directed to the major promoter of the c-myc gene.Nucleic acids research · 2002Article
- Stabilisation of TG- and AG-containing antiparallel DNA triplexes by triplex-binding ligands.Nucleic acids research · 2001Article
- Detection of competing DNA structures by thermal gradient gel electrophoresis: from self-association to triple helix formation by (G,A)-containing oligonucleotides.Nucleic acids research · 2001Article
- Optimization of alternate-strand triple helix formation at the 5"-TpA-3" and 5"-ApT-3" junctions.Nucleic acids research · 1999Article
- Factors influencing the extent and selectivity of alkylation within triplexes by reactive G/A motif oligonucleotides.Nucleic acids research · 1997Article
- Triple helix formation with purine-rich phosphorothioate-containing oligonucleotides covalently linked to an acridine derivative.Nucleic acids research · 1997Article
- Investigation of the formation and intracellular stability of purine.(purine/pyrimidine) triplexes.Nucleic acids research · 1997Article
- Potassium-resistant triple helix formation and improved intracellular gene targeting by oligodeoxyribonucleotides containing 7-deazaxanthine.Nucleic acids research · 1997Article
- A new approach to overcome potassium-mediated inhibition of triplex formation.Nucleic acids research · 1996Article
- Effect of third strand composition on the triple helix formation: purine versus pyrimidine oligodeoxynucleotides.Nucleic acids research · 1996Article
- Positively charged oligonucleotides overcome potassium-mediated inhibition of triplex DNA formation.Nucleic acids research · 1996Article
- Facilitating oligonucleotide delivery: helping antisense deliver on its promise.Proceedings of the National Academy of Sciences of the United States of America · 1996Review
- Triplex formation with alpha anomers of purine-rich and pyrimidine-rich oligodeoxynucleotides.Nucleic acids research · 1995Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Competition between triplex formation with double-stranded DNA and oligonucleotide self-association was investigated in 23mer GA and GT oligonucleotides containing d(GA)5 or d(GT)5 repeats. Whereas triplex formation with GT oligonucleotides was diminished when temperature increased from 4 to 37 degrees C, triplex formation with GA oligonucleotides was enhanced when temperature increased within the same range due to the presence of competing intermolecular GA oligonucleotide self-structure. This self-structure was determined to be a homoduplex stabilized by the internal GA repeats. UV spectroscopy of these homoduplexes demonstrated a single sharp transition with rapid kinetics (Tm = 38.5-43.5 degrees C over strand concentrations of 0.5-4 microM, respectively, with transition enthalpy, delta H = -89 +/- 7 kcal/mol) in 10 mM MgCl2, 100 mM NaCl, pH 7.0. Homoduplex formation was strongly stabilized by multivalent cations (spermine > Mg2+ = Ca2+) and destabilized by low concentrations of monovalent cations (K+ = Li+ = Na+) in the presence of divalent cations. However, unlike GA or GT oligonucleotide-containing triplexes, the homoduplex formed even in the absence of multivalent cations, stabilized by only moderate concentrations of monovalent cations (Li+ > Na+ > K+). Through the development of multiple equilibrium states and the resulting depletion of free oligonucleotide, it was found that the presence of competing self-structure could decrease triplex formation under a variety of experimental conditions.
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