ArticleThe Journal of clinical investigation1995
Fluid absorption in isolated perfused colonic crypts.
Article in The Journal of clinical investigation, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
33 citing papers in PubMed, 137 citations in OpenAlex.
- Ion transport and epithelial barrier dysfunction in experimental models of ulcerative colitis.American journal of physiology. Gastrointestinal and liver physiology · 2025Review
- From Crypts to Cancer: A Holistic Perspective on Colorectal Carcinogenesis and Therapeutic Strategies.International journal of molecular sciences · 2024Review
- Electroacupuncture Improving Intestinal Barrier Function in Rats with Irritable Bowel Syndrome Through Regulating Aquaporins.Digestive diseases and sciences · 2024Article
- Nutritional Considerations in Colorectal Surgery in Diverting Ileostomy Patients: A Review.Cureus · 2023Review
- The effect of claudin-15 deletion on cationic selectivity and transport in paracellular pathways of the cecum and large intestine.Scientific reports · 2023Article
- Human Colonoid-Myofibroblast Coculture for Study of Apical NaInternational journal of molecular sciences · 2023Article
- Review
- SLC9 Gene Family: Function, Expression, and Regulation.Comprehensive Physiology · 2018Review
- Differential DNA methylation patterns of homeobox genes in proximal and distal colon epithelial cells.Physiological genomics · 2016Article
- Human Enteroids as a Model of Upper Small Intestinal Ion Transport Physiology and Pathophysiology.Gastroenterology · 2016Article
- An investigation into the relationship between small intestinal fluid secretion and systemic arterial blood pressure in the anesthetized rat.Physiological reports · 2015Article
- Evidence from simultaneous intracellular- and surface-pH transients that carbonic anhydrase II enhances CO2 fluxes across Xenopus oocyte plasma membranes.American journal of physiology. Cell physiology · 2014Article
- Activation of AMPK inhibits cholera toxin stimulated chloride secretion in human and murine intestine.PloS one · 2013Article
- Evidence of a rudimentary colon in the elasmobranch, Leucoraja erinacea.Development genes and evolution · 2012Article
- Review
- Acetylcholine-induced activation of M3 muscarinic receptors stimulates robust matrix metalloproteinase gene expression in human colon cancer cells.American journal of physiology. Gastrointestinal and liver physiology · 2009Article
- Calcium-sensing receptor abrogates secretagogue- induced increases in intestinal net fluid secretion by enhancing cyclic nucleotide destruction.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Role of calcium and other trace elements in the gastrointestinal physiology.World journal of gastroenterology · 2006Review
- Chloride secretion in a morphologically differentiated human colonic cell line that expresses the epithelial Na+ channel.The Journal of physiology · 2004Article
- Oxygen consumption and chloride secretion in rat distal colon isolated mucosa.Digestive diseases and sciences · 2003Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
A spatial segregation of ion transport processes between crypt and surface epithelial cells is well-accepted and integrated into physiological and pathophysiological paradigms of small and large intestinal function: Absorptive processes are believed to be located in surface (and villous) cells, whereas secretory processes are believed to be present in crypt cells. Validation of this model requires direct determination of fluid movement in intestinal crypts. This study describes the adaptation of techniques from renal tubule microperfusion to hand-dissect and perfuse single, isolated crypts from rat distal colon to measure directly fluid movement. Morphologic analyses of the isolated crypt preparation revealed no extraepithelial cellular elements derived from the lamina propria, including myofibroblasts. In the basal state, crypts exhibited net fluid absorption (mean net fluid movement = 0.34 +/- 0.01 nl.mm-1.min-1), which was Na+ and partially HCO3- dependent. Addition of 1 mM dibutyryl-cyclic AMP, 60 nM vasoactive intestinal peptide, or 0.1 mM acetylcholine to the bath (serosal) solution reversibly induced net fluid secretion (net fluid movement approximately -0.35 +/- 0.01 nl.mm-1.min-1). These observations permit speculation that absorption is a constitutive transport function in crypt cells and that secretion by crypt cells is regulated by one or more neurohumoral agonists that are released in situ from lamina propria cells. The functional, intact polarized crypt described here that both absorbs and secretes will permit future studies that dissect the mechanisms that govern fluid and electrolyte movement in the colonic crypt.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.