ArticleThe Journal of clinical investigation1995
Acid extrusion is induced by osteoclast attachment to bone. Inhibition by alendronate and calcitonin.
Article in The Journal of clinical investigation, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
15 citing papers in PubMed, 1 synthesis or guideline pooled it, 111 citations in OpenAlex.
- Evidence-based guidelines for the treatment of postmenopausal osteoporosis: a consensus document of the Belgian Bone Club.Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA · 2005Guideline
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- Role of osteoclasts in regulating hematopoietic stem and progenitor cells.World journal of orthopedics · 2013Review
- Bisphosphonates: effects on osteoblast.European journal of clinical pharmacology · 2012Review
- Assay of in vitro osteoclast activity on dentine, and synthetic calcium phosphate bone substitutes.Journal of materials science. Materials in medicine · 2012Article
- Effects of ibandronate-hydroxyapatite on resorptive activity of osteoclasts.Archives of medical science : AMS · 2011Article
- The role of bisphosphonates in diseases of childhood.European journal of pediatrics · 2003Review
- Mechanism of action of bisphosphonates.Current osteoporosis reports · 2003Review
- Calcitonin inhibits proton extrusion in resorbing rat osteoclasts via protein kinase A.Pflugers Archiv : European journal of physiology · 2003Article
- Drugs in development: bisphosphonates and metalloproteinase inhibitors.Arthritis research & therapy · 2003Review
- Bisphosphonates in the treatment of metastatic breast cancer.Journal of mammary gland biology and neoplasia · 2001Review
- Pharmacokinetics of alendronate.Clinical pharmacokinetics · 1999Review
- Review
- Bisphosphonates: mechanisms of action.The Journal of clinical investigation · 1996Review
- Relationship between bisphosphonate concentration and osteoclast activity and viability.In vitro cellular & developmental biology. AnimalArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Acid extrusion is essential for osteoclast (OC) activity. We examined Na+ and HCO3(-)-independent H+ extrusion in rat- and mouse OCs by measuring intracellular pH (pHi) changes, with the pHi indicator BCECF (biscarboxyethyl-5-(6) carboxyfluorescein) after H+ loading with an ammonium pulse. 90% of OCs attached to glass do not possess HCO3- and Na(+)-independent H(+)-extrusion (rate of pHi recovery = 0.043 +/- 0.007 (SEM) pH U/min, n = 26). In contrast, in OCs attached to bone, the pHi recovery rate is 0.228 +/- 0.011 pHi U/min, n = 25. OCs on bone also possess a NH(4+)-permeable pathway not seen on glass. The bone-induced H+ extrusion was inhibited by salmon calcitonin (10(-8) M, for 2 h), and was not present after pretreating the bone slices with the aminobisphosphonate alendronate (ALN). At ALN levels of 0.22 nmol/mm2 bone, H+ extrusion was virtually absent 12 h after cell seeding (0.004 +/- 0.002 pH U/min) and approximately 50% inhibition was observed at 0.022 pmol ALN/mm2 bone. The Na(+)-independent H+ extrusion was not inhibited by bafilomycin A1 (up to 10(-7) M), although a bafilomycin A1 (10(-8) M)-sensitive H+ pump was present in membrane vesicles isolated from these osteoclasts. These findings indicate that Na(+)-independent acid extrusion is stimulated by osteoclast attachment to bone and is virtually absent when bone is preincubated with ALN, or when osteoclasts are treated with salmon calcitonin.
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