ArticleThe Journal of clinical investigation1995
Activation of protein kinase A acutely inhibits and phosphorylates Na/H exchanger NHE-3.
Article in The Journal of clinical investigation, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 93 citations in OpenAlex.
- NaInternational journal of molecular sciences · 2023Review
- Identification of a Primary Renal ATCirculation research · 2020Article
- Article
- Defective Renal Angiotensin III and ATJournal of the American Heart Association · 2019Article
- AT2 Receptor Activation Prevents Sodium Retention and Reduces Blood Pressure in Angiotensin II-Dependent Hypertension.Circulation research · 2016Article
- Molecular mechanisms and regulation of urinary acidification.Comprehensive Physiology · 2014Review
- Na+/H+ and Na+/NH+4 activities of zebrafish NHE3b expressed in Xenopus oocytes.American journal of physiology. Regulatory, integrative and comparative physiology · 2014Article
- HK-2 human renal proximal tubule cells as a model for G protein-coupled receptor kinase type 4-mediated dopamine 1 receptor uncoupling.Hypertension (Dallas, Tex. : 1979) · 2010Article
- BetaPix up-regulates Na+/H+ exchanger 3 through a Shank2-mediated protein-protein interaction.The Journal of biological chemistry · 2010Article
- NHE3 function and phosphorylation are regulated by a calyculin A-sensitive phosphatase.American journal of physiology. Renal physiology · 2010Article
- Luminal Na(+)/H (+) exchange in the proximal tubule.Pflugers Archiv : European journal of physiology · 2009Review
- Regulation of sodium pump endocytosis by cardiotonic steroids: Molecular mechanisms and physiological implications.Pathophysiology : the official journal of the International Society for Pathophysiology · 2007Article
- Na+/H+ exchangers in renal regulation of acid-base balance.Seminars in nephrology · 2006Review
- NHERF and regulation of the renal sodium-hydrogen exchanger NHE3.Pflugers Archiv : European journal of physiology · 2005Review
- Molecular aspects of acute inhibition of Na(+)-H(+) exchanger NHE3 by A(2)-adenosine receptor agonists.The Journal of physiology · 2002Article
- Basolateral Na(+)/HCO(3)(-) cotransport activity is regulated by the dissociable Na(+)/H(+) exchanger regulatory factor.The Journal of clinical investigation · 1999Article
- Adenosine inhibits the transfected Na+-H+ exchanger NHE3 in Xenopus laevis renal epithelial cells (A6/C1).The Journal of physiology · 1999Article
- Renal Na/H exchanger NHE-3 and Na-PO4 cotransporter NaPi-2 protein expression in glucocorticoid excess and deficient states.Journal of the American Society of Nephrology : JASN · 1998Article
- Structure-function of recombinant Na/H exchanger regulatory factor (NHE-RF).The Journal of clinical investigation · 1998Article
- cAMP-mediated inhibition of the epithelial brush border Na+/H+ exchanger, NHE3, requires an associated regulatory protein.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
In the mammalian renal proximal tubule, protein kinase A (PKA) plays an important role in mediating hormonal regulation of apical membrane Na/H exchanger activity. This exchanger is likely encoded by NHE-3. The present studies examined regulation of NHE-3 by PKA. NHE-3 was stably expressed in Na/H exchanger-deficient fibroblasts (AP-1/NHE-3 cells). PKA activation (0.1 mM 8-BrcAMP x 20 min) inhibited NHE-3 activity by 39% (P < 0.01) with no change in NHE-3 protein abundance in the plasma membrane. To define the structural requirements for PKA-mediated inhibition, full-length NHE-3 and a cytoplasmic domain-truncated mutant (NHE-3 delta cyto) were expressed in Xenopus laevis oocytes. 8-BrcAMP inhibited NHE-3 activity by 27% (P < 0.05), an effect that was blocked by 10(-7) M PKA inhibitor peptide. NHE-3 delta cyto had baseline activity similar to that of full-length NHE-3 but its activity was not regulated by 8-BrcAMP. The purified recombinant cytoplasmic domain of NHE-3 was phosphorylated in vitro by the catalytic subunit of PKA on serine residues. In AP-1/NHE-3 cells, NHE-3 was immunoprecipitated as a approximately 87-kD phosphoprotein. Addition of 0.1 mM 8-BrcAMP increased the phosphocontent of NHE-3 by threefold. In summary, acute activation of PKA inhibits NHE-3 activity, an effect that is likely mediated by phosphorylation of its cytoplasmic domain.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.