ArticleThe Journal of cell biology1995
Sequences, structural models, and cellular localization of the actin-related proteins Arp2 and Arp3 from Acanthamoeba.
Article in The Journal of cell biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
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Who cites it
60 citing papers in PubMed, 191 citations in OpenAlex.
- Article
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- The WAVE complex drives the morphogenesis of the photoreceptor outer segment cilium.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Article
- Role of ARP2/3 Complex-Driven Actin Polymerization in RSV Infection.Pathogens (Basel, Switzerland) · 2021Review
- Review
- Cellulose fibrils formation and organisation of cytoskeleton during encystment are essential for Acanthamoeba cyst wall architecture.Scientific reports · 2019Article
- From solution to surface to filament: actin flux into branched networks.Biophysical reviews · 2018Review
- Actin polymerization is stimulated by actin cross-linking protein palladin.The Biochemical journal · 2016Article
- The actin seed.Nature reviews. Molecular cell biology · 2016Article
- Arp2/3 complex function in the epidermis.Tissue barriers · 2014Article
- Article
- Arp2/3-mediated actin-based motility: a tail of pathogen abuse.Cell host & microbe · 2013Review
- Purification of native Arp2/3 complex from bovine thymus.Methods in molecular biology (Clifton, N.J.) · 2013Article
- Functional characterization of the human α-cardiac actin mutations Y166C and M305L involved in hypertrophic cardiomyopathy.Cellular and molecular life sciences : CMLS · 2012Article
- Actin engine in immunological synapse.Immune network · 2012Article
- Three-dimensional reconstructions of Arp2/3 complex with bound nucleation promoting factors.The EMBO journal · 2012Article
- Opportunities and successes in the search for plasmodesmal proteins.Protoplasma · 2011Review
- Adenomatous polyposis coli protein nucleates actin assembly and synergizes with the formin mDia1.The Journal of cell biology · 2010Article
- The p40/ARPC1 subunit of Arp2/3 complex performs multiple essential roles in WASp-regulated actin nucleation.The Journal of biological chemistry · 2010Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
We cloned and sequenced the two actin-related proteins (Arps) present in the profilin-binding complex of Acanthamoeba (Machesky, L. M., S. J. Atkinson, C. Ampe, J. Vandekerckhove, and T. D. Pollard. 1994, J. Cell Biol. 127:107-115). The sequence of Arp2 is more similar to other Arp2s than to actin, while the sequence of Arp3 is more similar to other Arp3s than to actin. Phylogenetic analysis of all known Arps demonstrates that most group into three major families, which are likely to be shared across all eukaryotic phyla. Together with conventional actins, the Arps form a larger family distinct from structurally related ATPases such as Hsp70's and sugar kinases. Atomic models of the Arps based on their sequences and the structure of actin provide some clues about function. Both Arps have atoms appropriately placed to bind ATP and divalent cation. Arp2, but not Arp3, has a conserved profilin-binding site. Neither Arp has the residues required to copolymerize with actin, but an Arp heterodimer present in the profilin-binding complex might serve as a pointed end nucleus for actin polymerization. Both Acanthamoeba Arps are soluble in cell homogenates, and both are concentrated in the cortex of Acanthamoeba. The cellular concentrations are 1.9 microM Arp2 and 5.1 microM Arp3, substoichiometric to actin (200 microM) but comparable to many actin-binding proteins.
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