ArticleJournal of bacteriology1995
Characterization of the polyketide synthase gene (pksL1) required for aflatoxin biosynthesis in Aspergillus parasiticus.
Article in Journal of bacteriology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 100 citations in OpenAlex.
- Are CurrentMycobiology · 2023Article
- Geographical Discrimination in Curcuminoids Content of Turmeric Assessed by Rapid UPLC-DAD Validated Analytical Method.Molecules (Basel, Switzerland) · 2019Article
- Aflatoxins: Implications on Health.Indian journal of clinical biochemistry : IJCB · 2017Review
- Phylogenetic and Structural Analysis of Polyketide Synthases in Aspergilli.Evolutionary bioinformatics online · 2016Article
- Bioinformatical analysis of the sequences, structures and functions of fungal polyketide synthase product template domains.Scientific reports · 2015Article
- The non-metabolizable glucose analog D-glucal inhibits aflatoxin biosynthesis and promotes kojic acid production in Aspergillus flavus.BMC microbiology · 2014Article
- Next-generation sequencing approach for connecting secondary metabolites to biosynthetic gene clusters in fungi.Frontiers in microbiology · 2014Review
- Navigating the fungal polyketide chemical space: from genes to molecules.The Journal of organic chemistry · 2012Article
- A resource for the in silico identification of fungal polyketide synthases from predicted fungal proteomes.Molecular plant pathology · 2012Article
- Acyl carrier protein structural classification and normal mode analysis.Protein science : a publication of the Protein Society · 2012Article
- Unraveling polyketide synthesis in members of the genus Aspergillus.Applied microbiology and biotechnology · 2010Review
- Towards prediction of metabolic products of polyketide synthases: an in silico analysis.PLoS computational biology · 2009Article
- The effect of temperature on Natural Antisense Transcript (NAT) expression in Aspergillus flavus.Current genetics · 2008Article
- Analysis of the dermatophyte Trichophyton rubrum expressed sequence tags.BMC genomics · 2006Article
- Biosynthesis of dothistromin.Mycopathologia · 2006Review
- Cyclo(L-leucyl-L-prolyl) produced by Achromobacter xylosoxidans inhibits aflatoxin production by Aspergillus parasiticus.Applied and environmental microbiology · 2004Article
- Novel polyketide synthase from Nectria haematococca.Applied and environmental microbiology · 2004Article
- Clustered pathway genes in aflatoxin biosynthesis.Applied and environmental microbiology · 2004Review
- Phylogenomic analysis of type I polyketide synthase genes in pathogenic and saprobic ascomycetes.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Dothistroma pini, a forest pathogen, contains homologs of aflatoxin biosynthetic pathway genes.Applied and environmental microbiology · 2002Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Aflatoxins are potent toxic and carcinogenic compounds, produced by Aspergillus parasiticus and A. flavus as secondary metabolites. In this research, a polyketide synthase gene (pksL1), the key gene for aflatoxin biosynthesis initiation in A. parasiticus, has been functionally identified and molecularly characterized. PCR-derived DNA probes were used to find the pksL1 gene from subtracted, aflatoxin-related clones. Gene knockout experiments generated four pksL1 disruptants which lost both the ability to produce aflatoxins B1, B2, and G1 and the ability to accumulate norsolorinic acid and all other intermediates of the aflatoxin biosynthetic pathway. A pksL1 DNA probe detected a 6.6-kb poly(A)+ RNA transcript in Northern (RNA) hybridizations. This transcript, associated with aflatoxin production, exhibited a regulated expression that was influenced by growth phase, medium composition, and culture temperature. DNA sequencing of pksL1 revealed an open reading frame for a polypeptide (PKSL1) of 2,109 amino acids. Sequence analysis further recognized four functional domains in PKSL1, acyl carrier protein, beta-ketoacyl-acyl carrier protein synthase, acyltransferase, and thioesterase, all of which are usually present in polyketide synthases and fatty acid synthases. On the basis of these results, we propose that pksL1 encodes the polyketide synthase which synthesizes the backbone polyketide and initiates aflatoxin biosynthesis. In addition, the transcript of pksL1 exhibited heterogeneity at the polyadenylation site similar to that of plant genes.
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