ArticleInfection and immunity1995
Mapping of the delayed-type hypersensitivity-inducing epitope of secreted protein MPT64 from Mycobacterium tuberculosis.
Article in Infection and immunity, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Screening and preclinical assessment of novelFrontiers in immunology · 2025Article
- Predominantly Orphan Secretome in the Lung PathogenMicroorganisms · 2024Article
- Proteomic analysis of purified protein derivative of Mycobacterium tuberculosis.Clinical proteomics · 2013Article
- Purified protein derivatives of tuberculin--past, present, and future.FEMS immunology and medical microbiology · 2012Review
- Use of recombinant purified protein derivative (PPD) antigens as specific skin test for tuberculosis.The Indian journal of medical research · 2012Article
- Three protein cocktails mediate delayed-type hypersensitivity responses indistinguishable from that elicited by purified protein derivative in the guinea pig model of Mycobacterium tuberculosis infection.Infection and immunity · 2011Article
- Assessing the serodiagnostic potential of 35 Mycobacterium tuberculosis proteins and identification of four novel serological antigens.Journal of clinical microbiology · 2005Article
- Specific delayed-type hypersensitivity responses to ESAT-6 identify tuberculosis-infected cattle.Journal of clinical microbiology · 2003Article
- Responses of bovine WC1(+) gammadelta T cells to protein and nonprotein antigens of Mycobacterium bovis.Infection and immunity · 2002Article
- Comparative immune response to PE and PE_PGRS antigens of Mycobacterium tuberculosis.Infection and immunity · 2001Article
- Diagnosis of tuberculosis based on the two specific antigens ESAT-6 and CFP10.Clinical and diagnostic laboratory immunology · 2000Article
- Functional domains present in the mycobacterial hemagglutinin, HBHA.Journal of bacteriology · 1999Article
- Differential T-cell recognition of native and recombinant Mycobacterium tuberculosis GroES.Infection and immunity · 1999Article
- Use of Mycobacterium tuberculosis complex-specific antigen cocktails for a skin test specific for tuberculosis.Infection and immunity · 1998Article
- Delayed-type hypersensitivity responses to ESAT-6 and MPT64 from Mycobacterium tuberculosis in the guinea pig.Infection and immunity · 1998Article
- MTC28, a novel 28-kilodalton proline-rich secreted antigen specific for the Mycobacterium tuberculosis complex.Infection and immunity · 1997Article
- Predominant recognition of the ESAT-6 protein in the first phase of interferon with Mycobacterium bovis in cattle.Infection and immunity · 1997Article
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5 authors.
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No grant is acknowledged in the PubMed record.
Abstract
The gene encoding the immunogenic protein MPT64 found in culture filtrates of Mycobacterium tuberculosis H37Rv was expressed in Escherichia coli K-12 and purified as a recombinant protein. The purified recombinant MPT64 elicited delayed-type hypersensitivity (DTH) in outbred guinea pigs sensitized with Mycobacterium bovis BCG Tokyo. The skin reactions were comparable to those obtained with native MPT64. No skin reactions were observed when either recombinant MPT64 or native MPT64 was used in guinea pigs sensitized with M. bovis BCG Danish 1331. Amino- and carboxy-terminal deletion mutants of MPT64 were purified as fusion proteins for the mapping of DTH-inducing epitopes on recombinant MPT64 by use of the guinea pig skin test model. The part of the molecule responsible for the biological activity was located at the carboxy-terminal end. Further studies with overlapping synthetic peptides have pinpointed the biological activity at a single DTH-inducing epitope consisting of 15 residues between amino acids Gly-173 and Ala-187. Screening by PCR of 56 clinical isolates of M. tuberculosis from Danish and Tanzanian patients demonstrated the presence of mpt64 in all of the strains. These results point to MPT64 as a possible candidate for a skin test reagent specific for diagnosis of human tuberculosis.
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