ArticleImmunogenetics1995
Analysis of human VH gene repertoire expression in peripheral CD19+ B cells.
Article in Immunogenetics, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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Who cites it
10 citing papers in PubMed, 35 citations in OpenAlex.
- Article
- Simple paired heavy- and light-chain antibody repertoire sequencing using endoplasmic reticulum microsomes.Genome medicine · 2018Article
- Multiple myeloma shows no intra-disease clustering of immunoglobulin heavy chain genes.Haematologica · 2012Article
- Efficient generation of monoclonal antibodies from single human B cells by single cell RT-PCR and expression vector cloning.Journal of immunological methods · 2008Article
- Evidence of biased immunoglobulin variable gene usage in highly stable B-cell chronic lymphocytic leukemia.Leukemia · 2004Article
- Molecular determinants of the human antibody response to HIV-1: implications for disease control.Journal of clinical immunology · 2001Article
- Third complementarity-determining region of mutated VH immunoglobulin genes contains shorter V, D, J, P, and N components than non-mutated genes.Immunology · 2001Article
- Ig heavy chain CDR3 size diversities are similar after conventional peripheral blood and ex vivo expanded hematopoietic cell transplants.Bone marrow transplantation · 2001Article
- VH usage and somatic hypermutation in peripheral blood B cells of patients with rheumatoid arthritis (RA).Clinical and experimental immunology · 1998Article
- Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Using CD19 B-cell selection and polymerase chain reaction-amplified cDNA libraries, we analyzed the peripheral immunoglobulin heavy chain variable repertoire of three healthy adult donors. Here we report that most of the CD19+ circulating B cells expressed unmutated VH-D-JH rearrangements. By specific VH family hybridization, we show that VH gene family utilization in the periphery roughly corresponds to the complexity of these families in the germline and appears to be relatively constant among the analyzed subjects. However, sequence data of clones picked at random from one IgM cDNA library reveals that in spite of this "random" utilization, the VH gene expression in naive circulating B cells is highly biased towards the expression of a limited set of VH genes. As previously reported by others, this restricted mechanism is also found for the D and JH segments.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.