ArticleCell calcium1995
Possibility of simultaneously measuring low and high calcium concentrations using Fura-2 and lifetime-based sensing.
Article in Cell calcium, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 39 citations in OpenAlex.
- Excitation-Dependent pKa Extends the Sensing Range of Fluorescence Lifetime pH Sensors.Sensors (Basel, Switzerland) · 2024Article
- Combining nitric oxide and calcium sensing for the detection of endothelial dysfunction.Communications chemistry · 2023Review
- Fluorescence lifetime excitation cytometry by kinetic dithering.Electrophoresis · 2014Article
- Automated fluorescence lifetime imaging plate reader and its application to Förster resonant energy transfer readout of Gag protein aggregation.Journal of biophotonics · 2013Article
- Major translocation of calcium upon epidermal barrier insult: imaging and quantification via FLIM/Fourier vector analysis.Archives of dermatological research · 2011Article
- FLIM FRET technology for drug discovery: automated multiwell-plate high-content analysis, multiplexed readouts and application in situ.Chemphyschem : a European journal of chemical physics and physical chemistry · 2011Article
- The epidermal Ca(2+) gradient: Measurement using the phasor representation of fluorescent lifetime imaging.Biophysical journal · 2010Article
- Rapid frequency-domain FLIM spinning disk confocal microscope: lifetime resolution, image improvement and wavelet analysis.Journal of fluorescence · 2008Article
- A mathematical model of cardiocyte Ca(2+) dynamics with a novel representation of sarcoplasmic reticular Ca(2+) control.Biophysical journal · 2000Article
- Global analysis of fluorescence lifetime imaging microscopy data.Biophysical journal · 2000Article
- Potassium and sodium measurements at clinical concentrations using phase-modulation fluorometry.Sensors and actuators. B, Chemical · 1999Article
- Sodium Green as a potential probe for intracellular sodium imaging based on fluorescence lifetime.Analytical biochemistry · 1997Article
- Long-lifetime metal-ligand pH probe.Analytical biochemistry · 1997Article
- Fluorescence lifetime characterization of magnesium probes: Improvement of Mg(2+) dynamic range and sensitivity using phase-modulation fluorometry.Journal of fluorescence · 1996Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
We characterized the fluorescence probe Fura-2 for calcium measurements using frequency-domain phase-modulation fluorometry. By the use of different excitation wavelengths from 345 to 380 nm, the apparent calcium dissociation constants can be altered from 41 nM to 1.92 microM Ca2+. This change in apparent Kd results from changes in the relative extent of excitation of the calcium-bound and calcium-free forms, and the excitation wavelength-dependent contribution of each form to the intensity decay. These results indicate that lifetime-based measurements with Fura-2 can be used for imaging of calcium over a wide range of concentrations. An additional favorable feature of Fura-2 is that the calcium-free form can be almost exclusively excited at wavelength of 390 nm or longer, and can thus be used as a reference providing the lifetime in the absence of calcium, without removing the calcium. Additionally, exposure of Fura-2 to intense illumination shifts but does not distort the frequency response. For cellular imaging, these favorable properties of Fura-2 may allow calibration of the calcium concentrations without the use of ionophores.
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Registered trials
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