ArticleTransgenic research1995
An in vivo analysis of transcriptional elements in the mouse alpha-myosin heavy chain gene promoter.
Article in Transgenic research, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 40 citations in OpenAlex.
- ExcessiveCirculation · 2021Article
- Network-based predictions of in vivo cardiac hypertrophy.Journal of molecular and cellular cardiology · 2018Article
- Healing a Heart Through Genetic Intervention.Circulation research · 2016Article
- Giving credence to controls: Avoiding the false phenotype.Journal of molecular and cellular cardiology · 2015Article
- Gremlin 2 promotes differentiation of embryonic stem cells to atrial fate by activation of the JNK signaling pathway.Stem cells (Dayton, Ohio) · 2014Article
- Normal cardiac function in mice with supraphysiological cardiac creatine levels.American journal of physiology. Heart and circulatory physiology · 2014Article
- Tropomodulin isoforms regulate thin filament pointed-end capping and skeletal muscle physiology.The Journal of cell biology · 2010Article
- Reversibility of PRKAG2 glycogen-storage cardiomyopathy and electrophysiological manifestations.Circulation · 2008Article
- Diversity in transcriptional start site selection and alternative splicing affects the 5'-UTR of mouse striated muscle myosin transcripts.Journal of muscle research and cell motility · 2006Article
- Mice with cardiomyocyte-specific disruption of the endothelin-1 gene are resistant to hyperthyroid cardiac hypertrophy.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Cluster characterisation and temporal expression of porcine sarcomeric myosin heavy chain genes.Journal of muscle research and cell motility · 2003Article
- Disruption of integrin function in the murine myocardium leads to perinatal lethality, fibrosis, and abnormal cardiac performance.The American journal of pathology · 2001Article
- Altered focal adhesion regulation correlates with cardiomyopathy in mice expressing constitutively active rac1.The Journal of clinical investigation · 2000Article
- Transgenic over-expression of a motor protein at high levels results in severe cardiac pathology.Transgenic research · 1999Article
- Myofibril degeneration caused by tropomodulin overexpression leads to dilated cardiomyopathy in juvenile mice.The Journal of clinical investigation · 1998Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
During development in the murine ventricle, there is a switch in myosin heavy chain gene (MyHC) transcription. The beta-MyHC is expressed in the ventricles during foetal development, but is shut down at or around birth, at which time alpha-MyHC transcription is activated. This antithetical switch is thought to be mediated by circulating levels of thyroid hormone (TH) and both low and high affinity thyroid response elements (TREs) have been identified in the proximal promoter region of the murine alpha-MyHC. Myosin gene expression in the atria is relatively unaffected by the TH status. Previously, we used site-directed mutagenesis of the promoter in a transgenic analysis to define those elements responsible for high levels of transcription in vivo. These analyses focused on the role(s) of two cis elements, TRE1 and TRE2 that are located at -129 to -149 and -102 to -120, respectively, on the alpha-MyHC promoter. Although the elements' ablation had differential effects on transgene expression, neither single mutation abolished transgene expression completely. Here, we show that mutating both elements results in a complete inactivation of the transgene in both ventricles and atria under euthyroid conditions. However, expression still can be detected in the hyperthyroid state, implying that, although the TRE1 and TRE2 elements are critical elements for high levels of alpha-MyHC transcription in vivo, other promoter sites can mediate at least some degree of transcriptional activation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.