ArticleThe Plant cell1995
Composite structure of auxin response elements.
Article in The Plant cell, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 164 papers.
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Who cites it
164 citing papers in PubMed, 363 citations in OpenAlex.
- AUXIN RESPONSE FACTORs: from transcription factors to auxin effectors.The New phytologist · 2026Review
- Evolutionary origin of a new gene drives root growth in one desert poplar.The New phytologist · 2026Article
- ARF6 integrates auxin and gibberellin signaling to promote stone cell lignification in pear via the HB49-MYB169 module.The New phytologist · 2026Article
- A conserved ARF-DNA interface underlies auxin-triggered transcriptional response.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Synthesis and Degradation of the Phytohormone Indole-3-Acetic Acid by the Versatile BacteriumPlants (Basel, Switzerland) · 2024Article
- An auxin research odyssey: 1989-2023.The Plant cell · 2024Review
- The hormone regulatory mechanism underlying parthenocarpic fruit formation in tomato.Frontiers in plant science · 2024Review
- Enigmatic role of auxin response factors in plant growth and stress tolerance.Frontiers in plant science · 2024Review
- A non-canonicalFrontiers in plant science · 2024Article
- To bind or not to bind: how AUXIN RESPONSE FACTORs select their target genes.Journal of experimental botany · 2023Review
- Cooperative action of separate interaction domains promotes high-affinity DNA binding ofProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Divergent regulation of auxin responsive genes in root-knot and cyst nematodes feeding sites formed in Arabidopsis.Frontiers in plant science · 2023Article
- Application of data fusion modeling for the prediction of auxin response elements in Zea mays for food security purposes.Genomics & informatics · 2022Article
- On the trail of auxin: Reporters and sensors.The Plant cell · 2022Review
- AUXIN RESPONSE FACTOR7 integrates gibberellin and auxin signaling via interactions between DELLA and AUX/IAA proteins to regulate cambial activity in poplar.The Plant cell · 2022Article
- An Arabidopsis mutant deficient in phosphatidylinositol-4-phosphate kinases ß1 and ß2 displays altered auxin-related responses in roots.Scientific reports · 2022Article
- An in-frame deletion mutation in the degron tail of auxin coreceptorProceedings of the National Academy of Sciences of the United States of America · 2022Article
- A plastidial retrograde signal potentiates biosynthesis of systemic stress response activators.The New phytologist · 2022Article
- CIN-like TCP13 is essential for plant growth regulation under dehydration stress.Plant molecular biology · 2022Article
- Structural Aspects of Auxin Signaling.Cold Spring Harbor perspectives in biology · 2022Review
104 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The auxin-responsive soybean GH3 gene promoter is composed of multiple auxin response elements (AuxREs), and each AuxRE contributes incrementally to the strong auxin inducibility to the promoter. Two independent AuxREs of 25 bp (D1) and 32 bp (D4) contain the sequence TGTCTC. Results presented here show that the TGTCTC element in D1 and D4 is required but not sufficient for auxin inducibility in carrot protoplast transient expression assays. Additional nucleotides upstream of TGTCTC are also required for auxin inducibility. These upstream sequences showed constitutive activity and no auxin inducibility when part or all of the TGTCTC element was mutated or deleted. In D1, the constitutive element overlaps the 5' portion of TGTCTC; in D4, the constitutive element is separated from TGTCTC. An 11-bp element in D1, CCTCGTGTCTC, conferred auxin inducibility to a minimal cauliflower mosaic virus 35S promoter in transgenic tobacco seedlings as well as in carrot protoplasts (i.e., transient expression assays). Both constitutive elements bound specifically to plant nuclear proteins, and the constitutive element in D1 bound to a recombinant soybean basic leucine zipper transcription factor with G-box specificity. To demonstrate further the composite nature of AuxREs and the ability of the TGTCTC element to confer auxin inducibility, we created a novel AuxRE by placing a yeast GAL4 DNA binding site adjacent to the TGTCTC element. Expression of a GAL4-c-Rel transactivator in the presence of this novel AuxRE resulted in auxin-inducible expression. Our results indicate that at least some AuxREs have a composite structure consisting of a constitutive element adjacent to a conserved TGTCTC element that confers auxin inducibility.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.