ArticleMolecular biology of the cell1995
Phosphorylated and dephosphorylated linker histone H1 reside in distinct chromatin domains in Tetrahymena macronuclei.
Article in Molecular biology of the cell, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 41 citations in OpenAlex.
- A genetic screen for regulators of Drosophila histone H1 binding and chromosome structure in vivo.Genetics · 2026Article
- Phosphorylation of Human Retinoid X Receptor α at Serine 260 Impairs Its Subcellular Localization, Receptor Interaction, Nuclear Mobility, and 1α,25-Dihydroxyvitamin D3-dependent DNA Binding in Ras-transformed Keratinocytes.The Journal of biological chemistry · 2017Article
- Single-Molecule Studies of the Linker Histone H1 Binding to DNA and the Nucleosome.Biochemistry · 2016Article
- Pin1 promotes histone H1 dephosphorylation and stabilizes its binding to chromatin.The Journal of cell biology · 2013Article
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- Unphosphorylated H1 is enriched in a specific region of the promoter when CDC2 is down-regulated during starvation.Molecular and cellular biology · 2007Article
- The H1 phosphorylation state regulates expression of CDC2 and other genes in response to starvation in Tetrahymena thermophila.Molecular and cellular biology · 2005Article
- The dynamic mobility of histone H1 is regulated by cyclin/CDK phosphorylation.Molecular and cellular biology · 2003Article
- Spatiotemporal sites of DNA replication in macro- and micronuclei of the ciliate Paramecium caudatum.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2003Article
- Histone H1 phosphorylation by Cdk2 selectively modulates mouse mammary tumor virus transcription through chromatin remodeling.Molecular and cellular biology · 2001Article
- An HP1-like protein is missing from transcriptionally silent micronuclei of Tetrahymena.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Prolonged glucocorticoid exposure dephosphorylates histone H1 and inactivates the MMTV promoter.The EMBO journal · 1998Article
- Phosphorylation of linker histone is associated with transcriptional activation in a normally silent nucleus.The Journal of cell biology · 1996Article
- Activation of an MAP kinase cascade leads to Sir3p hyperphosphorylation and strengthens transcriptional silencing.The Journal of cell biology · 1996Article
- Increased histone H1 phosphorylation and relaxed chromatin structure in Rb-deficient fibroblasts.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Phosphorylated and dephosphorylated isoforms of Tetrahymena macronuclear H1 were separated from each other by cation-exchange high performance liquid chromatography and used to generate a pairwise set of antisera that discriminate the phosphorylation state of this linker histone. Affinity-purified antibodies from each sera recognize appropriate H1 isoforms and stain macronuclei under appropriate physiological conditions. Immunogold localizations demonstrate that phosphorylated and dephosphorylated H1 localize nonrandomly in distinct subdomains of macronuclear chromatin. Dephosphorylated H1 is strongly enriched in the electron-dense chromatin bodies that punctuate macronuclear chromatin. In contrast, phosphorylated H1 isoforms, as well as an evolutionarily conserved H2A.F/Z-like variant (hv1) believed to function in the establishment of transcriptionally competent chromatin, are modestly enriched at the periphery of chromatin bodies and in the surrounding euchromatin. Using antibodies against TATA-binding protein, we show that transcriptionally active chromatin lies outside of the chromatin bodies in an area relatively devoid of H1. Antibodies against general core histones are more or less evenly distributed across these domains. Together, these data are consistent with a model in which phosphorylation of H1, perhaps in association with hv1, loosens the binding of H1 in chromatin leading to chromatin decondensation as part of a first-step mechanism in gene activation. In contrast, our data support the view that dephosphorylation of this linker histone facilitates or stabilizes condensed, transcriptionally silent chromatin.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.