ArticleMolecular biology of the cell1995
The rho-GAP encoded by BEM2 regulates cytoskeletal structure in budding yeast.
Article in Molecular biology of the cell, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 64 citations in OpenAlex.
- Integrative Phosphoproteomic and Proteomic Analysis ofJournal of proteome research · 2025Article
- Article
- Compensation for the absence of the catalytically active half of DNA polymerase ε in yeast by positively selected mutations in CDC28.Genetics · 2021Article
- Inhibition of Cdc42 during mitotic exit is required for cytokinesis.The Journal of cell biology · 2013Article
- Control of polarized growth by the Rho family GTPase Rho4 in budding yeast: requirement of the N-terminal extension of Rho4 and regulation by the Rho GTPase-activating protein Bem2.Eukaryotic cell · 2013Article
- The Rho1 GTPase-activating protein CgBem2 is required for survival of azole stress in Candida glabrata.The Journal of biological chemistry · 2011Article
- Phosphorylation of Bem2p and Bem3p may contribute to local activation of Cdc42p at bud emergence.The EMBO journal · 2007Article
- Dosage rescue by UBC4 restores cell wall integrity in Saccharomyces cerevisiae lacking the myosin type II gene MYO1.Yeast (Chichester, England) · 2007Article
- Epsin N-terminal homology domains perform an essential function regulating Cdc42 through binding Cdc42 GTPase-activating proteins.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Comparison of cell wall localization among Pir family proteins and functional dissection of the region required for cell wall binding and bud scar recruitment of Pir1p.Eukaryotic cell · 2005Article
- The Dictyostelium genome encodes numerous RasGEFs with multiple biological roles.Genome biology · 2005Article
- Motifs, themes and thematic maps of an integrated Saccharomyces cerevisiae interaction network.Journal of biology · 2005Article
- The PXL1 gene of Saccharomyces cerevisiae encodes a paxillin-like protein functioning in polarized cell growth.Molecular biology of the cell · 2004Article
- The Rho-GAP Bem2p plays a GAP-independent role in the morphogenesis checkpoint.The EMBO journal · 2002Article
- Osmotic stress signaling and osmoadaptation in yeasts.Microbiology and molecular biology reviews : MMBR · 2002Review
- Induction of a mitosis delay and cell lysis by high-level secretion of mouse alpha-amylase from Saccharomyces cerevisiae.Applied and environmental microbiology · 2001Article
- Ras regulates the polarity of the yeast actin cytoskeleton through the stress response pathway.Molecular biology of the cell · 2001Article
- Suppressors of mdm20 in yeast identify new alleles of ACT1 and TPM1 predicted to enhance actin-tropomyosin interactions.Genetics · 2000Article
- Bni1p regulates microtubule-dependent nuclear migration through the actin cytoskeleton in Saccharomyces cerevisiae.Molecular and cellular biology · 1999Article
- The yeast ser/thr phosphatases sit4 and ppz1 play opposite roles in regulation of the cell cycle.Molecular and cellular biology · 1999Article
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2 authors at 1 institution in 1 country.
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Abstract
Microfilaments are required for polarized growth and morphogenesis in Saccharomyces cerevisiae. To accomplish this, actin cables and patches are redistributed during the cell cycle to direct secretory components to appropriate sites for cell growth. A major component of actin cables is tropomyosin I, encoded by TPM1, that determines or stabilizes these structures. Disruption of TPM1 is not lethal but results in the loss of actin cables and confers a partial defect in polarized secretion. Using a synthetic lethal screen, we have identified seven mutations residing in six genes whose products are required in the absence of Tpm1p. Each mutant exhibited a morphological defect, suggesting a functional link to the actin cytoskeleton. Complementation cloning of one mutation revealed that it lies in BEM2, which encodes a GTPase-activating protein for the RHO1 product. bem2 mutations also show synthetic lethality with rho1 and mutations in certain other cytoskeletal genes (ACT1, MYO1, MYO2, and SAC6) but not with mutations in several noncytoskeletal genes. These data therefore provide a genetic link between the GAP encoded by BEM2 and the functional organization of microfilaments. In addition, we show that bem2 mutations confer benomyl sensitivity and have abnormal microtubule arrays, suggesting that the BEM2 product may also be involved directly or indirectly in regulating microtubule function.
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