ArticleMolecular biology of the cell1995
Human RNA polymerase II subunit hsRPB7 functions in yeast and influences stress survival and cell morphology.
Article in Molecular biology of the cell, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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28 citing papers in PubMed, 72 citations in OpenAlex.
- Formation of S. pombe Erh1 homodimer mediates gametogenic gene silencing and meiosis progression.Scientific reports · 2020Article
- Article
- Intrinsically disordered proteins in the nucleus of human cells.Biochemistry and biophysics reports · 2015Article
- The RNA Pol II sub-complex hsRpb4/7 is required for viability of multiple human cell lines.Protein & cell · 2012Article
- mRNA imprinting: Additional level in the regulation of gene expression.Cellular logistics · 2011Article
- Integrative structure modeling of macromolecular assemblies from proteomics data.Molecular & cellular proteomics : MCP · 2010Article
- Basal transcription machinery: role in regulation of stress response in eukaryotes.Journal of biosciences · 2007Review
- The RNA polymerase II subunit Rpb4p mediates decay of a specific class of mRNAs.Genes & development · 2005Article
- Domainal organization of the lower eukaryotic homologs of the yeast RNA polymerase II core subunit Rpb7 reflects functional conservation.Nucleic acids research · 2004Article
- Identification of the RNA polymerase II subunit hsRPB7 as a novel target of the von Hippel-Lindau protein.The EMBO journal · 2003Article
- Architecture of initiation-competent 12-subunit RNA polymerase II.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- An Rpb4/Rpb7-like complex in yeast RNA polymerase III contains the orthologue of mammalian CGRP-RCP.Molecular and cellular biology · 2003Article
- The Ess1 prolyl isomerase is required for growth and morphogenetic switching in Candida albicans.Genetics · 2002Article
- Article
- Suprabasal overexpression of the hsRPB7 gene in psoriatic epidermis as identified by a reverse transcriptase-polymerase chain reaction differential display model comparing psoriasis plaque tissue with peritonsillar mucosa.The American journal of pathology · 2001Article
- A human RNA polymerase II subunit is encoded by a recently generated multigene family.BMC molecular biology · 2001Article
- Multiple mechanisms of suppression circumvent transcription defects in an RNA polymerase mutant.Molecular and cellular biology · 2000Article
- The Ess1 prolyl isomerase is linked to chromatin remodeling complexes and the general transcription machinery.The EMBO journal · 2000Article
- The Saccharomyces telomere-binding protein Cdc13p interacts with both the catalytic subunit of DNA polymerase alpha and the telomerase-associated est1 protein.Genes & development · 2000Article
- The Rpb4 subunit of fission yeast Schizosaccharomyces pombe RNA polymerase II is essential for cell viability and similar in structure to the corresponding subunits of higher eukaryotes.Molecular and cellular biology · 1999Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Using a screen to identify human genes that promote pseudohyphal conversion in Saccharomyces cerevisiae, we obtained a cDNA encoding hsRPB7, a human homologue of the seventh largest subunit of yeast RNA polymerase II (RPB7). Overexpression of yeast RPB7 in a comparable strain background caused more pronounced cell elongation than overexpression of hsRPB7. hsRPB7 sequence and function are strongly conserved with its yeast counterpart because its expression can rescue deletion of the essential RPB7 gene at moderate temperatures. Further, immuno-precipitation of RNA polymerase II from yeast cells containing hsRPB7 revealed that the hsRPB7 assembles the complete set of 11 other yeast subunits. However, at temperature extremes and during maintenance at stationary phase, hsRPB7-containing yeast cells lose viability rapidly, stress-sensitive phenotypes reminiscent of those associated with deletion of the RPB4 subunit with which RPB7 normally complexes. Two-hybrid analysis revealed that although hsRPB7 and RPB4 interact, the association is of lower affinity than the RPB4-RPB7 interaction, providing a probable mechanism for the failure of hsRPB7 to fully function in yeast cells at high and low temperatures. Finally, surprisingly, hsRPB7 RNA in human cells is expressed in a tissue-specific pattern that differs from that of the RNA polymerase II largest subunit, implying a potential regulatory role for hsRPB7. Taken together, these results suggest that some RPB7 functions may be analogous to those possessed by the stress-specific prokaryotic sigma factor rpoS.
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