ArticleMolecular biology of the cell1995
ida4-1, ida4-2, and ida4-3 are intron splicing mutations affecting the locus encoding p28, a light chain of Chlamydomonas axonemal inner dynein arms.
Article in Molecular biology of the cell, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 73 citations in OpenAlex.
- Article
- Article
- Heme-binding protein CYB5D1 is a radial spoke component required for coordinated ciliary beating.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Composition and function of ciliary inner-dynein-arm subunits studied in Chlamydomonas reinhardtii.Cytoskeleton (Hoboken, N.J.) · 2021Review
- Asymmetries in the cilia ofPhilosophical transactions of the Royal Society of London. Series B, Biological sciences · 2020Review
- Axonemal Dynein Arms.Cold Spring Harbor perspectives in biology · 2016Review
- The Chlamydomonas mutant pf27 reveals novel features of ciliary radial spoke assembly.Cytoskeleton (Hoboken, N.J.) · 2013Article
- New mutations in flagellar motors identified by whole genome sequencing in Chlamydomonas.Cilia · 2013Article
- The nexin-dynein regulatory complex subunit DRC1 is essential for motile cilia function in algae and humans.Nature genetics · 2013Article
- An axonemal PP2A B-subunit is required for PP2A localization and flagellar motility.Cytoskeleton (Hoboken, N.J.) · 2011Article
- A novel ankyrin-repeat protein interacts with the regulatory proteins of inner arm dynein f (I1) of Chlamydomonas reinhardtii.Cell motility and the cytoskeleton · 2009Article
- Chlamydomonas outer arm dynein alters conformation in response to Ca2+.Molecular biology of the cell · 2007Article
- A methionine synthase homolog is associated with secretory vesicles in tobacco pollen tubes.Planta · 2005Article
- The human dynein intermediate chain 2 gene (DNAI2): cloning, mapping, expression pattern, and evaluation as a candidate for primary ciliary dyskinesia.Human genetics · 2000Article
- The 9 + 2 axoneme anchors multiple inner arm dyneins and a network of kinases and phosphatases that control motility.The Journal of cell biology · 2000Review
- Insights into the structural organization of the I1 inner arm dynein from a domain analysis of the 1beta dynein heavy chain.Molecular biology of the cell · 2000Article
- Loss-of-function mutations in a human gene related to Chlamydomonas reinhardtii dynein IC78 result in primary ciliary dyskinesia.American journal of human genetics · 1999Article
- Domains in the 1alpha dynein heavy chain required for inner arm assembly and flagellar motility in Chlamydomonas.The Journal of cell biology · 1999Article
- Cytoplasmic dynein heavy chain 1b is required for flagellar assembly in Chlamydomonas.Molecular biology of the cell · 1999Article
- Distinct mutants of retrograde intraflagellar transport (IFT) share similar morphological and molecular defects.The Journal of cell biology · 1998Article
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2 authors at 1 institution in 1 country.
Funding
Abstract
We recently determined the nucleotide sequence of the gene encoding p28, a light chain of inner dynein arms of Chlamydomonas axonemes. Here, we show that p28 is the protein encoded by the IDA4 locus. p28, and the dynein heavy chains normally associated with it, are completely absent from the flagella and cell bodies of three allelic strains of ida4, named ida4-1, ida4-2, and ida4-3. We determined the nucleotide sequence of the three alleles of the p28 gene and found in each case a single nucleotide change, affecting the splice sites of the first, second, and fourth introns, respectively. Reverse transcriptase-polymerase chain reaction amplification of RNAs prepared from ida4 cells confirmed that these mutations prevent the correct splicing of the affected introns, thereby blocking the synthesis of full-length p28. These are the first intron splicing mutations described in Chlamydomonas and the first inner dynein arm mutations characterized at the molecular level. The absence in ida4 axonemes of the dynein heavy chains normally found in association with p28 suggests that p28 is necessary for stable assembly of a subset of inner dynein arms or for the binding of these arms to the microtubule doublets.
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