ArticleBritish journal of cancer1995
8-Chloro-cAMP induces apoptotic cell death in a human mammary carcinoma cell (MCF-7) line.
Article in British journal of cancer, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 49 citations in OpenAlex.
- PDE2A Is Indispensable for Mouse Liver Development and Hematopoiesis.International journal of molecular sciences · 2020Article
- N-3 poly-unsaturated fatty acids shift estrogen signaling to inhibit human breast cancer cell growth.PloS one · 2012Article
- Evaluation of human first trimester decidual and telomerase-transformed endometrial stromal cells as model systems of in vitro decidualization.Reproductive biology and endocrinology : RB&E · 2011Article
- AMID mediates adenosine-induced caspase-independent HuH-7 cell apoptosis.Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology · 2011Article
- HTR1B as a risk profile maker in psychiatric disorders: a review through motivation and memory.European journal of clinical pharmacology · 2010Review
- Marine benthic diatoms contain compounds able to induce leukemia cell death and modulate blood platelet activity.Marine drugs · 2009Article
- Camptothecin and khat (Catha edulis Forsk.) induced distinct cell death phenotypes involving modulation of c-FLIPL, Mcl-1, procaspase-8 and mitochondrial function in acute myeloid leukemia cell lines.Molecular cancer · 2009Article
- Extracellular adenosine induces apoptosis in Caco-2 human colonic cancer cells by activating caspase-9/-3 via A(2a) adenosine receptors.Journal of gastroenterology · 2009Article
- Khat (Catha edulis)-induced apoptosis is inhibited by antagonists of caspase-1 and -8 in human leukaemia cells.British journal of cancer · 2004Article
- Caspase I-related protease inhibition retards the execution of okadaic acid- and camptothecin-induced apoptosis and PAI-2 cleavage, but not commitment to cell death in HL-60 cells.British journal of cancer · 1999Article
- Biochemical and molecular mechanisms regulating apoptosis.Molecular and cellular biochemistry · 1998Review
- Molecular Events as Targets of Anticancer Drug Therapy.Pathology oncology research : POR · 1997Article
- Role of protein kinase activity in apoptosis.Experientia · 1996Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
8-Cl-cAMP and 8-NH2-cAMP induced MCF-7 cell death. The type(s) of cell death were studied in more detail and compared with the cell death type (apoptosis) induced by okadaic acid, an inhibitor of serine/threonine phosphatases. By morphological criteria dying cells showed loss of cell-cell interactions and microvilli, condensation of nuclear chromatin and segregation of cytoplasmic organelles. By in situ nick end-labelling, using digoxigenin-conjugated dUTP as probe, a large fraction of 8-Cl-cAMP, 8-NH2-cAMP and 8-Cl-adenosine-exposed cells stained positively in the advanced stages of death. In the early phase of chromatin condensation the cells stained negatively. Specific (internucleosomal) DNA fragmentation was not observed. The MCF-7 cell death induced by 8-Cl-cAMP and 8-NH2-cAMP was not mediated by activation of the cAMP kinase since more stable cAMP analogues (8-CPT-cAMP and N6-benzoyl-cAMP) or forskolin failed to induce death. Furthermore, 8-Cl-cAMP action was counteracted by adenosine deaminase and 3-isobutyl-1-methylxanthine, and mimicked by 8-Cl-adenosine, a major metabolite of 8-Cl-cAMP. It is concluded that 8-Cl- and 8-NH2-cAMP can induce morphological and biochemical effects resembling apoptotic cell death in MCF-7 cells through their conversion into potent cytotoxic metabolite(s).
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