ArticleProceedings of the National Academy of Sciences of the United States of America1995
3T3 fibroblasts transfected with a cDNA for mitochondrial aspartate aminotransferase express plasma membrane fatty acid-binding protein and saturable fatty acid uptake.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 152 citations in OpenAlex.
- Fatty Acid Transport and Signaling: Mechanisms and Physiological Implications.Annual review of physiology · 2023Review
- A Cancer Cell-Intrinsic GOT2-PPARδ Axis Suppresses Antitumor Immunity.Cancer discovery · 2022Article
- Measurement of long-chain fatty acid uptake into adipocytes.Methods in enzymology · 2014Article
- Age-related expression profile of the SLC27A1 gene in chicken tissues.Molecular biology reports · 2011Article
- Molecular modeling and functional confirmation of a predicted fatty acid binding site of mitochondrial aspartate aminotransferase.Journal of molecular biology · 2011Article
- Palmitoylation of ketogenic enzyme HMGCS2 enhances its interaction with PPARalpha and transcription at the Hmgcs2 PPRE.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2010Article
- Stranger in a strange land: roles of glycogen turnover in adipose tissue metabolism.Molecular and cellular endocrinology · 2010Review
- Regulatable fatty acid transport mechanisms are central to the pathophysiology of obesity, fatty liver, and metabolic syndrome.Hepatology (Baltimore, Md.) · 2008Review
- Protein-mediated Fatty Acid Uptake in the Heart.Current cardiology reviews · 2008Article
- Fatty acid binding protein facilitates sarcolemmal fatty acid transport but not mitochondrial oxidation in rat and human skeletal muscle.The Journal of physiology · 2007Article
- Subcellular localization of fumarase in mammalian cells and tissues.Histochemistry and cell biology · 2007Article
- Fatty acid handling protein expression in adipose tissue, fatty acid composition of adipose tissue and serum, and markers of insulin resistance.European journal of clinical nutrition · 2006Article
- Tissue-specific and fatty acid transporter-specific changes in heart and soleus muscle over a 1-yr period.Molecular and cellular biochemistry · 2006Article
- Increased hepatocellular uptake of long chain fatty acids occurs by different mechanisms in fatty livers due to obesity or excess ethanol use, contributing to development of steatohepatitis in both settings.Transactions of the American Clinical and Climatological Association · 2005Article
- Regulation of cardiac long-chain fatty acid and glucose uptake by translocation of substrate transporters.Pflugers Archiv : European journal of physiology · 2004Review
- Transmembrane movement of exogenous long-chain fatty acids: proteins, enzymes, and vectorial esterification.Microbiology and molecular biology reviews : MMBR · 2003Review
- An aspartate aminotransferase of Wolbachia endobacteria from Onchocerca volvulus is recognized by IgG1 antibodies from residents of endemic areas.Parasitology research · 2003Article
- Sulfo-N-succinimidyl esters of long chain fatty acids specifically inhibit fatty acid translocase (FAT/CD36)-mediated cellular fatty acid uptake.Molecular and cellular biochemistry · 2002Review
- Regulation of fatty acid transport and membrane transporters in health and disease.Molecular and cellular biochemistry · 2002Review
- A new concept of cellular uptake and intracellular trafficking of long-chain fatty acids.Lipids · 2001Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
To explore the relationship between mitochondrial aspartate aminotransferase (mAspAT; EC 2.6.1.1) and plasma membrane fatty acid-binding protein (FABPpm) and their role in cellular fatty acid uptake, 3T3 fibroblasts were cotransfected with plasmid pMAAT2, containing a full-length mAspAT cDNA downstream of a Zn(2+)-inducible metallothionein promoter, and pFR400, which conveys methotrexate resistance. Transfectants were selected in methotrexate, cloned, and exposed to increasing methotrexate concentrations to induce gene amplification. Stably transfected clones were characterized by Southern blotting; those with highest copy numbers of pFR400 alone (pFR400) or pFR400 and pMAAT2 (pFR400/pMAAT2) were expanded for further study. [3H]Oleate uptake was measured in medium containing 500 microM bovine serum albumin and 125-1000 microM total oleate (unbound oleate, 18-420 nM) and consisted of saturable and nonsaturable components. pFR400/pMAAT2 cells exhibited no increase in the rate constant for nonsaturable oleate uptake or in the uptake rate of [14C]octanoate under any conditions. By contrast, Vmax (fmol/sec per 50,000 cells) of the saturable oleate uptake component increased 3.5-fold in pFR400/pMAAT2 cells compared to pFR400, with a further 3.2-fold increase in the presence of Zn2+. Zn2+ had no effect in pFR400 controls (P > 0.5). The overall increase in Vmax between pFR400 and pFR400/pMAAT2 in the presence of Zn2+ was 10.4-fold (P < 0.01) and was highly correlated (r = 0.99) with expression of FABPpm in plasma membranes as determined by Western blotting. Neither untransfected 3T3 nor pFR400 cells expressed cell surface FABPpm detectable by immunofluorescence. By contrast, plasma membrane immunofluorescence was detected in pFR400/pMAAT2 cells, especially if cultured in 100 microM Zn2+. The data support the dual hypotheses that mAspAT and FABPpm are identical and mediate saturable long-chain free fatty acid uptake.
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