ArticleProceedings of the National Academy of Sciences of the United States of America1995
A general strategy for producing conditional alleles of Src-like tyrosine kinases.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 78 citations in OpenAlex.
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- Chemical activation of divergent protein tyrosine phosphatase domains with cyanine-based biarsenicals.Scientific reports · 2019Article
- Chemically induced proximity in biology and medicine.Science (New York, N.Y.) · 2018Review
- Selective Targeting of Cells via Bispecific Molecules That Exploit Coexpression of Two Intracellular Proteins.ACS chemical biology · 2015Article
- Direct Chemical Activation of a Rationally Engineered Signaling Enzyme.Chembiochem : a European journal of chemical biology · 2015Article
- Protein design: Past, present, and future.Biopolymers · 2015Review
- Fatty acyl chain-dependent but charge-independent association of the SH4 domain of Lck with lipid membranes.Journal of biosciences · 2013Article
- Engineering the ABA plant stress pathway for regulation of induced proximity.Science signaling · 2011Article
- The promise of optogenetics in cell biology: interrogating molecular circuits in space and time.Nature methods · 2011Article
- Conditionally controlling nuclear trafficking in yeast by chemical-induced protein dimerization.Nature protocols · 2010Article
- Biomolecular engineering of intracellular switches in eukaryotes.Journal of drug delivery science and technology · 2010Article
- Rapid inactivation of proteins by rapamycin-induced rerouting to mitochondria.Developmental cell · 2010Article
- Brought to life: targeted activation of enzyme function with small molecules.Journal of chemical biology · 2009Article
- A novel conditional Akt 'survival switch' reversibly protects cells from apoptosis.Gene therapy · 2002Article
- Controlled dimerization of ErbB receptors provides evidence for differential signaling by homo- and heterodimers.Molecular and cellular biology · 1999Article
- Targeted expansion of genetically modified bone marrow cells.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Synthetic activation of caspases: artificial death switches.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Latent membrane protein 1 of Epstein-Barr virus mimics a constitutively active receptor molecule.The EMBO journal · 1997Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The Src-like tyrosine kinases require membrane localization for transformation and probably for their normal role in signal transduction. We utilized this characteristic to prepare Src-like tyrosine kinases that can be readily activated with the rationally designed chemical inducer of dimerization FK1012. Dimerization of cytoplasmic Src-like tyrosine kinases was not sufficient for signaling, but their recruitment to the plasma membrane led to the rapid activation of transcription factors identical to those regulated by crosslinking the antigen receptor. Moreover, recruitment of activated Src-like kinases to the membrane replaced signaling by the T-lymphocyte antigen receptor complex, leading to the activation of both the Ras/protein kinase C and Ca2+/calcineurin pathways normally activated by antigen receptor signaling. Since these chemical inducers of dimerization are cell permeable, this approach permits the production of conditional alleles of any of the Src-like tyrosine kinases, thereby allowing a delineation of their developmental roles.
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