ArticleProceedings of the National Academy of Sciences of the United States of America1995
Fc epsilon RI-mediated recruitment of p53/56lyn to detergent-resistant membrane domains accompanies cellular signaling.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 98 papers.
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Who cites it
98 citing papers in PubMed, 297 citations in OpenAlex.
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- Boxcar Imaging FCS Reveals Membrane Raft Stabilization Kinetics in Antigen-Stimulated Mast Cells.bioRxiv : the preprint server for biology · 2025Article
- Critical roles of plasma membrane dynamics and heterogeneity at the early stage of antigen-stimulated FcεRI signaling in mast cells.Biochemical Society transactions · 2025Review
- Synergy between membrane topography and domains to control signaling protein localization in mast cells facilitates their activation.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Lipid-driven interleaflet coupling of plasma membrane order regulates FcεRI signaling in mast cells.Biophysical journal · 2024Article
- Sorting of complex sphingolipids within the cellular endomembrane systems.Frontiers in cell and developmental biology · 2024Review
- Biomimetic Lipid Raft: Domain Stability and Interaction with Physiologically Active Molecules.Advances in experimental medicine and biology · 2024Review
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- Transbilayer Coupling of Lipids in Cells Investigated by Imaging Fluorescence Correlation Spectroscopy.The journal of physical chemistry. B · 2022Article
- Alteration of cholesterol distribution at the plasma membrane of cancer cells: From evidence to pathophysiological implication and promising therapy strategy.Frontiers in physiology · 2022Review
- Lipid-based and protein-based interactions synergize transmembrane signaling stimulated by antigen clustering of IgE receptors.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Innate immune receptor clustering and its role in immune regulation.Journal of cell science · 2021Review
- The desmosome as a model for lipid raft driven membrane domain organization.Biochimica et biophysica acta. Biomembranes · 2020Review
- The FcεRIβ homologue, MS4A4A, promotes FcεRI signal transduction and store-operated CaCellular signalling · 2020Article
- Direct Inhibition of the Allergic Effector Response by Raw Cow's Milk-An Extensive In Vitro Assessment.Cells · 2020Article
- Conceptual Evolution of Cell Signaling.International journal of molecular sciences · 2019Review
- Interaction of DJ-1 with Lyn is essential for IgE-mediated stimulation of human mast cells.The Journal of allergy and clinical immunology · 2018Article
- Binding of canonical Wnt ligands to their receptor complexes occurs in ordered plasma membrane environments.The FEBS journal · 2017Article
38 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Detergent-resistant plasma membrane structures, such as caveolae, have been implicated in signalling, transport, and vesicle trafficking functions. Using sucrose gradient ultracentrifugation, we have isolated low-density, Triton X-100-insoluble membrane domains from RBL-2H3 mucosal mast cells that contain several markers common to caveolae, including a src-family tyrosine kinase, p53/56lyn. Aggregation of Fc epsilon RI, the high-affinity IgE receptor, causes a significant increase in the amount of p53/56lyn associated with these low-density membrane domains. Under our standard conditions for lysis, IgE-Fc epsilon RI fractionates with the majority of the solubilized proteins, whereas aggregated receptor complexes are found at a higher density in the gradient. Stimulated translocation of p53/56lyn is accompanied by increased tyrosine phosphorylation of several proteins in the low-density membrane domains as well as enhanced in vitro tyrosine kinase activity toward these proteins and an exogenous substrate. With a lower detergent-to-cell ratio during lysis, significant Fc epsilon RI remains associated with these membrane domains, consistent with the ability to coimmunoprecipitate tyrosine kinase activity with Fc epsilon RI under similar lysis conditions [Pribluda, V. S., Pribluda, C. & Metzger, H. (1994) Proc. Natl. Acad. Sci. USA 91, 11246-11250]. These results indicate that specialized membrane domains may be directly involved in the coupling of receptor aggregation to the activation of signaling events.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.