ArticleProceedings of the National Academy of Sciences of the United States of America1995
Targeted disruption of vinculin genes in F9 and embryonic stem cells changes cell morphology, adhesion, and locomotion.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 78 papers.
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Who cites it
78 citing papers in PubMed, 204 citations in OpenAlex.
- Beyond Transconductance: Cell-Polymer Coupling Determines Fidelity in Action Potential Recording via Electrolyte-Gated Polymer Transistors.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Molecular basis and cellular functions of vinculin-actin directional catch bonding.Nature communications · 2023Article
- Vinculin Y822 is an important determinant of ligand binding.Journal of cell science · 2023Article
- Complete Model of Vinculin Suggests the Mechanism of Activation by Helical Super-Bundle Unfurling.The protein journal · 2022Article
- Adhesion strength and anti-tumor agents regulate vinculin of breast cancer cells.Frontiers in oncology · 2022Article
- An Optimized O9-1/Hydrogel System for Studying Mechanical Signals in Neural Crest Cells.Journal of visualized experiments : JoVE · 2021Article
- Vinculin-mediated axon growth requires interaction with actin but not talin in mouse neocortical neurons.Cellular and molecular life sciences : CMLS · 2021Article
- The Cryogenic Electron Microscopy Structure of the Cell Adhesion Regulator Metavinculin Reveals an Isoform-Specific Kinked Helix in Its Cytoskeleton Binding Domain.International journal of molecular sciences · 2021Article
- Article
- The Role of Hypoxia in Corneal Extracellular Matrix Deposition and Cell Motility.Anatomical record (Hoboken, N.J. : 2007) · 2020Article
- Vinculin expression in non-small cell lung cancer.The Journal of international medical research · 2020Article
- Distinct Binding Modes of Vinculin Isoforms Underlie Their Functional Differences.Structure (London, England : 1993) · 2019Article
- Cardiomyopathy Mutations in Metavinculin Disrupt Regulation of Vinculin-Induced F-Actin Assemblies.Journal of molecular biology · 2019Article
- Vinculin and metavinculin exhibit distinct effects on focal adhesion properties, cell migration, and mechanotransduction.PloS one · 2019Article
- Vinculin and the mechanical response of adherent fibroblasts to matrix deformation.Scientific reports · 2018Article
- Article
- Geometric constraints of endothelial cell migration on electrospun fibres.Scientific reports · 2018Article
- Myosin X is recruited to nascent focal adhesions at the leading edge and induces multi-cycle filopodial elongation.Scientific reports · 2017Article
- Vinculin forms a directionally asymmetric catch bond with F-actin.Science (New York, N.Y.) · 2017Article
- Single and collective cell migration: the mechanics of adhesions.Molecular biology of the cell · 2017Article
18 more citing papers are in PubMed but not listed here.
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Vinculin, a major constituent of focal adhesions and zonula adherens junctions, is thought to be involved in linking the microfilaments to areas of cell-substrate and cell-cell contacts. To test the role of vinculin in cell adhesion and motility, we used homologous recombination to generate F9 embryonal carcinoma and embryonic stem cell clones homozygous for a disrupted vinculin gene. When compared to wild-type cells, vinculin-mutant cells displayed a rounder morphology and a reduced ability to adhere and spread on plastic or fibronectin. Decreased adhesion of the mutant cells was associated with a reduction in lamellipodial extensions, as observed by time-lapse video microscopy. The locomotive activities of control F9 and the vinculin-null cells were compared in two assays. Loss of vinculin resulted in a 2.4-fold increase in cell motility. These results demonstrate an important role for vinculin in determining cell shape, adhesion, surface protrusive activity, and cell locomotion.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.