ArticleProceedings of the National Academy of Sciences of the United States of America1995
A nuclear gene of eubacterial origin in Euglena gracilis reflects cryptic endosymbioses during protist evolution.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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43 citing papers in PubMed, 172 citations in OpenAlex.
- Mapping the proteome landscape inPlant signaling & behavior · 2026Article
- Splice site diversity and abundance of noncanonical introns in diplonemids (Diplonemea, Euglenozoa).RNA (New York, N.Y.) · 2025Article
- Glycosylated proteins in the protozoan alga Euglena gracilis: a proteomic approach.FEMS microbiology letters · 2023Article
- Promoter-Bound Full-Length Intronic Circular RNAs-RNA Polymerase II Complexes Regulate Gene Expression in the Human ParasiteNon-coding RNA · 2022Article
- Fungal Mitogenomes: Relevant Features to Planning Plant Disease Management.Frontiers in microbiology · 2020Review
- Physiology, phylogeny, early evolution, and GAPDH.Protoplasma · 2017Review
- Intermediate introns in nuclear genes of euglenids - are they a distinct type?BMC evolutionary biology · 2016Article
- Distribution of conventional and nonconventional introns in tubulin (α and β) genes of euglenids.Molecular biology and evolution · 2014Article
- Article
- Multiple inter-kingdom horizontal gene transfers in the evolution of the phosphoenolpyruvate carboxylase gene family.PloS one · 2012Article
- A possible role for short introns in the acquisition of stroma-targeting peptides in the flagellate Euglena gracilis.DNA research : an international journal for rapid publication of reports on genes and genomes · 2010Article
- A split and rearranged nuclear gene encoding the iron-sulfur subunit of mitochondrial succinate dehydrogenase in Euglenozoa.BMC research notes · 2009Article
- Article
- A complex and punctate distribution of three eukaryotic genes derived by lateral gene transfer.BMC evolutionary biology · 2007Article
- Analysis of Euglena gracilis plastid-targeted proteins reveals different classes of transit sequences.Eukaryotic cell · 2006Article
- A "green" phosphoribulokinase in complex algae with red plastids: evidence for a single secondary endosymbiosis leading to haptophytes, cryptophytes, heterokonts, and dinoflagellates.Journal of molecular evolution · 2006Article
- Unusual features of fibrillarin cDNA and gene structure in Euglena gracilis: evolutionary conservation of core proteins and structural predictions for methylation-guide box C/D snoRNPs throughout the domain Eucarya.Nucleic acids research · 2005Article
- Phylogenomic evidence supports past endosymbiosis, intracellular and horizontal gene transfer in Cryptosporidium parvum.Genome biology · 2004Article
- Evolution of energy metabolism and its compartmentation in Kinetoplastida.Kinetoplastid biology and disease · 2003Article
- Distribution and phylogenies of enzymes of the Embden-Meyerhof-Parnas pathway from archaea and hyperthermophilic bacteria support a gluconeogenic origin of metabolism.Archaea (Vancouver, B.C.) · 2003Review
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Genes for glycolytic and Calvin-cycle glyceraldehyde-3-phosphate dehydrogenase (GAPDH) of higher eukaryotes derive from ancient gene duplications which occurred in eubacterial genomes; both were transferred to the nucleus during the course of endosymbiosis. We have cloned cDNAs encoding chloroplast and cytosolic GAPDH from the early-branching photosynthetic protist Euglena gracilis and have determined the structure of its nuclear gene for cytosolic GAPDH. The gene contains four introns which possess unusual secondary structures, do not obey the GT-AG rule, and are flanked by 2- to 3-bp direct repeats. A gene phylogeny for these sequences in the context of eubacterial homologues indicates that euglenozoa, like higher eukaryotes, have obtained their GAPDH genes from eubacteria via endosymbiotic (organelle-to-nucleus) gene transfer. The data further suggest that the early-branching protists Giardia lamblia and Entamoeba histolytica--which lack mitochondria--and portions of the trypanosome lineage have acquired GAPDH genes from eubacterial donors which did not ultimately give rise to contemporary membrane-bound organelles. Evidence that "cryptic" (possibly ephemeral) endosymbioses during evolution may have entailed successful gene transfer is preserved in protist nuclear gene sequences.
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