ArticleNucleic acids research1995
An intronic (A/U)GGG repeat enhances the splicing of an alternative intron of the chicken beta-tropomyosin pre-mRNA.
Article in Nucleic acids research, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
64 citing papers in PubMed, 114 citations in OpenAlex.
- Review
- A novel (ATC)Veterinary medicine and science · 2021Article
- Antisense oligonucleotide development for the selective modulation of CYP3A5 in renal disease.Scientific reports · 2021Article
- Intrinsic Regulatory Role of RNA Structural Arrangement in Alternative Splicing Control.International journal of molecular sciences · 2020Review
- G-tract RNA removes Polycomb repressive complex 2 from genes.Nature structural & molecular biology · 2019Article
- Novel splicing in IGFN1 intron 15 and role of stable G-quadruplex in the regulation of splicing in renal cell carcinoma.PloS one · 2018Article
- Computational Analysis of G-Quadruplex Forming Sequences across Chromosomes Reveals High Density Patterns Near the Terminal Ends.PloS one · 2016Article
- Identification and Validation of Evolutionarily Conserved Unusually Short Pre-mRNA Introns in the Human Genome.International journal of molecular sciences · 2015Article
- G-quadruplex formation enhances splicing efficiency of PAX9 intron 1.Human genetics · 2015Article
- A heroin addiction severity-associated intronic single nucleotide polymorphism modulates alternative pre-mRNA splicing of the μ opioid receptor gene OPRM1 via hnRNPH interactions.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- Divergence of Drosophila melanogaster repeatomes in response to a sharp microclimate contrast in Evolution Canyon, Israel.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Optimal antisense target reducing INS intron 1 retention is adjacent to a parallel G quadruplex.Nucleic acids research · 2014Article
- Alternative splicing of TAF6: downstream transcriptome impacts and upstream RNA splice control elements.PloS one · 2014Article
- The high kinetic stability of a G-quadruplex limits hnRNP F qRRM3 binding to G-tract RNA.Nucleic acids research · 2013Article
- Article
- Frequent gain and loss of intronic splicing regulatory elements during the evolution of vertebrates.Genome biology and evolution · 2012Article
- G Run-mediated recognition of proteolipid protein and DM20 5' splice sites by U1 small nuclear RNA is regulated by context and proximity to the splice site.The Journal of biological chemistry · 2011Article
- A network of conserved co-occurring motifs for the regulation of alternative splicing.Nucleic acids research · 2010Article
- PIN1 gene variants in Alzheimer's disease.BMC medical genetics · 2009Article
- Identification and characterization of nucleolin as a c-myc G-quadruplex-binding protein.The Journal of biological chemistry · 2009Article
4 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Computer analysis of human intron sequences have revealed a 50 nucleotide (nt) GC-rich region downstream of the 5' splice site; the trinucleotide GGG occurs almost four times as frequently as it would in a random sequence. The 5' part of a beta-tropomyosin intron exhibits six repetitions of the motif (A/U)GGG. In order to test whether these motifs play a role in the splicing process we have mutated some or all of them. Mutated RNAs show a lower in vitro splicing efficiency when compared with the wild-type, especially when all six motifs are mutated (> 70% inhibition). Assembly of the spliceosome complex B and, to a lesser extent, of the pre-spliceosome complex A also appears to be strongly affected by this mutation. A 55 kDa protein within HeLa cell nuclear extract is efficiently cross-linked to the G-rich region. This protein is present in the splicing complexes and its cross-linking to the pre-mRNA requires the presence of one or several snRNP. Altogether our results suggest that the G-rich sequences present in the 5' part of introns may act as an enhancer of the splicing reaction at the level of spliceosome assembly.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.